Content of oenothein B in willow herb depending on growing conditions
| Author(s) | |
|---|---|
Uminska, Kateryna | Zhytomyr Basic Pharmaceutical Professional College, Ukraine |
Kozurak, Alla | Carpathian Biosphere Reserve, Rakhiv, Zakarpattia Region, Ukraine |
Georgiyants, Victoriya | Department of Pharmaceutical Chemistry, National University of Pharmacy, Kharkiv, Ukraine |
Mykhailenko, Olha | Department of Pharmaceutical Chemistry, National University of Pharmacy, Kharkiv, Ukraine |
| Date Issued |
|---|
2022-02-18 |
ISBN 978-966-437-620-1 (повне зібрання) ISBN 978-966-437-621-8 (Том 1)
Bibliogr.: p. 145
Introduction. Chamerion angustifolium (L.) Holub (Onagraceae) is a representative of willow herb species found throughout lower temperate northern regions. Its use to treat prostatic adenoma has been reported. Willow herb has been traditionally used in folk medicine and currently it is a potential raw material for the production of phytopharmaceuticals. The aerial part of willow herb is used in folk medicine to treat a variety of ailments, including benign prostatic hyperplasia and associated urinary problems, rectal bleeding, to relieve menstrual disorders, and others [1]. The willow herb is rich in polyphenolic compounds such as flavonol-3-O-glycosides, phenolic acids and ellagitannins. Myricetin, quercetin, kaempferol and their various glycosides were dominant in samples of Epilobium species. The most biologically active compound is oenotein B, which has antioxidant properties and is responsible for the predominant amount of plant activity [2]. The aim of this work was to determine the highest amount of oenothein B in tested samples of willow herb, which was collected in different regions of Ukraine on the massive blooming phase. Materials and methods. The aim of this study was to perform a comparative analysis of the quantitative composition of oenothein B content using the HPLC method in Chamerion angustifolium (L.) Holub. herb harvested in different geographical areas of Ukraine. Sample 1 was collected in the Zakarpattia region, the Carpathian Mountains, Chornahora massif. Sample 2 was harvested in the Kharkiv region, Kachalivka village. Both samples were collected in June of 2019 (massive blooming phase). The extraction was performed in an ultrasonic bath for 30 minutes. Chromatographic separation was conducted in a Shimadzu Nexera X2 LC-30AD HPLC system using an ACE C18 column in a solvent system with 0.1% trifluoracetic acid in water under gradient increase elution of acetonitrile from 5 to 95%. Chromatograms were recorded at 265 nm. The identification of the compounds was based on the UV/MS spectral data as well as co-chromatography with the reference compound. The agrochemical analysis (the content of humus, рН, the content of P2O5, K2O, N) was carried out according to the well-known methods of DSTU 4289:2004, DSTU 4115-20002, DSTU 4115-2002, DSTU ISO 10390-2001 [3].. [...].