Total Phenolic Compounds of Potentilla Anserina (L.) Rydb. in Aerial Parts During Vegetation Period
Date Issued |
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2023-02-20 |
ISBN 978-966-437-658-4 (повне зібрання) ISBN 978-966-437-656-0 (Том 1)
Bibliogr.: p. 94
Introduction. Potentilla anserina (L.) Rydb. is a low-growing herbaceous plant in Rosaceae family and is prevalent on river shores and in grassy habitats such as meadows and road-sides throughout Europe, Siberia, the Far East and Central Asia. The plant is distributed across the temperate zone of the world [1]. The primary reasons that decoctions, tinctures, and infusions of P. anserina are used as a therapeutic raw material are its anti-diarrheic, spasmolytic properties, as well as antimicrobial and antiviral activities. The medicinal effect is determined by the tannins and flavonoids contained in the phytochemical composition of the plant [2]. The aim of the study was to evaluate the amount of phenolic compounds of Potentilla anserina during different vegetation period. Materials and methods. Aerial parts of P. anserina every two weeks in the months of June, July and August were collected from Northern part of Lithuania. In order to prepare the extracts, raw material was dried at room temperature, protected from direct sunlight and milled using the electric grinder. The ultrasonic extraction method and 20 mL of 70% acetone and 0.2 of dry milled matter were used to prepare the extracts. The total phenolic compounds of P. anserina were determined according to colorimetric Folin–Ciocalteu method [3]. Results and their discussion. In different vegetation periods the total phenolic content was in a range of 91.24 ± 2.46 GAE mg/g – 116.52 ± 17.32 GAE mg/g. The results have shown that the greatest amount of phenolic content was determined in July 116.52 ± 17.32 GAE mg/g during massive flowering period and the lowest was in August 91.24 ± 2.46 GAE mg/g at the end of vegetation. Conclusion. It is important to collect the plant during vegetation period. In this study it was determined that greatest amount of phenolic compounds were collected during the flowering.