Staphylococcus Hominis isolated from hospital patient resistance to antibiotics and resistance to antimicrobial genes
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2015-05-14 |
Abstract speech of authors were not edited.
The aim of this study was to determine antimicrobial resistance to antibiotics level Staphylococcus hominis isolated from hospital patients. One hundred twenty-five samples were tested for isolated Staphylococcus hominis (S. hominis). Isolation of staphylococci was performed using Mannitol Salt Agar and Plasmacoagulase test was performed as well. Species identification was performed using Microgen Staph ID (Microgen Bioproducts) as well as 16S rRNR gene sequencing. Antimicrobial susceptibility was performed using “Sensititre” (Trek Diagnostic Systems) plates. Interpretation of results were evaluated according to EUCAST recommendations. Genes encoding resistance to separate classes of antimicrobials were detected by PCR. 125 hospital patients were investigate. Thirty one isolates of Staphylococcus hominis was obtained from humans patients (24.8%). 26 (83.9%) S. hominis strains were obtained from 31 demonstrated resistance to one or more antibiotcs. The resistance of S. hominis isolates was detected to penicillin (77.41%) and ampicillin (61.2%), erythromycin (80.6%), tetracycline (48.3%), trimethoprim/sulfamethoxazole (25.8%), ciprofloxacin (25.8%), clindamycin (29.0%), gatifloxacin (22.5%), levofloxacin (19.3%), rifampin (9.6%) and gentamicin (6.4%). 11 (35.5%) isolated from S. hominis strains were resistant to methicillin, all strains were susceptible to vancomycin, quinupristin/dalfopristin, ceftriaxone, daptomycin and linezolid. All methicillin resistance S. hominis strains carried the mecA as well as blaZ genes. S. hominis strains resistant to tetracycline carried the tetK gene (32%), to macrolides - msrAB gene (19.4%). ermC gene was also prevalent in 9.6% isolates. Gentamicin resistant genes aac(6′)-Ie-aph(2")-Ia and aph(3′)-IIIa were detected in 3.2% S. hominis strains. Resistance to trimethoprim in S. hominis strains was encoded by dfrG (6.5%). The results suggest that clinical S. hominis strains are resistant to antibiotics. In spite that S. h