Arklių ciatostomų rūšys ir jų atsparumas fenbendazolui

Abstract

Eksperimentas atliktas SP UAB Vilniaus žirgynas, kuriame HKSS metodu buvo nustatytas strongilų atsparumas fenbendazolui. Eksperimentuota su 4 arkliais (trys bandomieji ir kontrolinis). Bandomieji arkliai dehelmintizuoti antihelmintiku fenbendazolu, skiriant po 7,5 mg/kg kūno masės. Po 13–14 parų jiems skirta ivermektino – po 0,2 mg/kg kūno masės. Kontrolinis arklys nedehelmintizuotas. Eksperimento metu buvo surinkta ir identifikuota 5491 ciatostomas. Nustatyta, kad tirtuose arkliuose parazitavo 13 rūšių ciatostomai. Identifikuotos ciatostomų rūšys priklausė 4 gentims. Po 4 rūšis radome Cylicocyclus ir Cylicostephanus, tris rūšis Coronocyclus ir dvi Cyathostomum gentyse. Gausiausios buvo Cylicocyclus nassatus ir Cyathostomum catinatum (42,18%; 22,73%) rūšys, nors gana gausiai aptikta Cylicocyclus leptostomus, Cylicostephanus longibursatus, Cylicocyclus ashworthi, Cylicostephanus goldi, Cyathostomum pateratum (13,6%; 7,79%; 7,76%; 3,15%; 2,17%). Kitų šešių rūšių (Coronocyclus labiatus, Cylicostephanus minutus, Coronocyclus labratus, Cylicocyclus insigne, Cylicostephanus calicatus, Coronocyclus coronatus) rasta tik po keletą individų. Atskiruose arkliuose parazitavo nuo 8 iki 10 ciatostomų rūšių. Nustatyta, kad Cylicocyclus ashworthi, Cylicocyclus nassatus, Cylicostephanus goldi parodė stipriai išreikštą atsparumą fenbendazolui (atitinkamai 54,69%; 53,02%; 34,10%). Cyathostomum catinatum buvo mažiau atspari (16,83%). Prie silpnai atsparių rūšių galima priskirti Cylicostephanus longibursatus ir Cyathostomum pateratum, kai tuo tarpu Cylicocyclus leptostomus buvo visiškai neatspari fenbendazolui. Apie kitų rūšių atsparumą sunku spręsti dėl mažo rastų ciatostomų skaičiaus. [...]. The experiment was conducted at the Lithuanian horse breeding farm Vilniaus žirgynas, where horse strongyles resistance to fenbendazoles was earlier detected by FECR test. The experiment included 4 horses, naturally infected with strongyles. The following anthelminthics were administered to three experimental horses: firstly Fenbendazole (7.5 mg /kg BW) and 13-14 days later – Ivermectin (0.2 mg/kg BW). One (control) horse was not treated. The total of 5491 cyathostomes were collected and identified during the experiment. It was determined that horses are hosts of 13 varieties of cyathostomes. The identified varieties belonged to four genera: 4 varieties from Cylicocyclus and Cylicostephanus, 3 varieties from Coronocyclus and two from Cyathostomum genera. Cylicocyclus nassatus and Cyathostomum catinatum (42.18%, 22.73%) were the dominant varieties though Cylicocyclus leptostomus, Cylicostephanus longibursatus, Cylicocyclus ashworthi, Cylicostephanus goldi, Cyathostomum pateratum (13.6%, 7.79%, 7.76%, 3.15%, 2.17%) were also present in abundance. The other six varieties (Coronocyclus labiatus, Cylicostephanus minutus, Coronocyclus labratus, Cylicocyclus insigne, Cylicostephanus calicatus, Coronocyclus coronatus) were found only as solitary individuals. Some horses were hosts of 8 to 10 varieties of cyathostomes. It was determined that Cylicocyclus ashworthi, Cylicocyclus nassatus, Cylicostephanus goldi had developed strong resistance to Fenbendazole (54.69%, 53.02%, 34.10% respectively). Cyathostomum catinatum was less resistant (16.83%). Cylicostephanus longibursatus and Cyathostomum pateratum can be attributed to weakly resistant varieties whereas Cylicocyclus leptostomus performed no resistance at all. The small number of detected other varieties is an insufficient basis for judgment about their resistance. [...].