Role of variants at SIRT1 gene in pituitary adenoma

Abstract

Background and Aim: Pituitary adenomas (PAs) accounts for approximately 10% off primary brain tumors. It is usually located in the front two-thirds of the pituitary gland and may extend into surrounding structures resulting a lot of complications: headache, visual impairment and behavioral changes (American brain tumor association, 2017). The present study aimed to determine the association between two sirtuin 1 (SIRT1) gene polymorphisms (rs4746720 and rs3740051) and PA development and investigate how they associated with gender. Materials and Methods: The study included 70 patients with a diagnosis of PA. The reference group involved 120 healthy subjects. The genotyping of SIRT1 rs3740051 and rs4746720 were performed using the real-time polymerase chain reaction method. The potential associations with single nucleotide polymorphisms (rs3740051, rs4746720) were evaluated for all patients. Results: Genotyping results revealed that rs4746720 AA variant was observed with a frequency of 100 % in all study groups. Statistical analysis of SIRT1 rs3740051 did not reveal significant differences in genotype (AA, AG, GG) distribution between PA and control groups: 78.6%, 18.6%, 2.8% vs. 85.0%, 14.2%, 0.8%, respectively (p=0.301). Analysis of genotype distribution between females and males did not exhibit any significant differences either: genotype (AA, AG, GG) distribution in PA and control females (79.0%, 18.4%, 2.6% vs. 87.5%, 10.9%, 1.6%, respectively, p=0.517) and in PA and control males (75.0%, 21.9%, 3.1% vs. 82.1%, 17.9%, 0%, respectively, p=0.359). Conclusions: The SIRT1 rs3740051 and rs4746720 polymorphisms were not associated with PA development.