microRNAs Are Abundant and Stable in Platelet-Rich Fibrin and Other Autologous Blood Products of Canines

Jasinevičiūtė, Indrė; Hasan, Nazmul; Grigas, Juozas; Pautienius, Arnoldas; Stankevičius, Arūnas; Žymantienė, Judita; Miura, Naoki

doi:10.3390/ijms24010770

Use this url to cite publication: https://hdl.handle.net/20.500.12512/116463

microRNAs Are Abundant and Stable in Platelet-Rich Fibrin and Other Autologous Blood Products of Canines

Details

Type of publication

Straipsnis Web of Science ir Scopus duomenų bazėje / Article in Web of Science and Scopus database (S1)

Author(s)

Author	Affiliation
Jasinevičiūtė, Indrė	VF Anatomijos ir fiziologijos katedra (13.03)
Hasan, Nazmul	Kagoshima University, Japan
Grigas, Juozas	VF Anatomijos ir fiziologijos katedra (13.03)	VF Mikrobiologijos ir virusologijos institutas (13.02)
Pautienius, Arnoldas	VF Anatomijos ir fiziologijos katedra (13.03)	VF Mikrobiologijos ir virusologijos institutas (13.02)
Stankevičius, Arūnas	VF Anatomijos ir fiziologijos katedra (13.03)
Žymantienė, Judita	VF Anatomijos ir fiziologijos katedra (13.03)
Miura, Naoki	Kagoshima University, Japan

Title

microRNAs Are Abundant and Stable in Platelet-Rich Fibrin and Other Autologous Blood Products of Canines

[en]

Publisher (trusted)

Multidisciplinary Digital Publishing Institute (MDPI AG)

Is Referenced by

Science Citation Index Expanded

Scopus

PubMed

PubMed Central

Date Issued

Date	Volume	Issue	Start Page	End Page
2023-01-01	24	1	1	10

Is part of

International Journal of Molecular Sciences

Version

Originalus / Original

Description

art. no. 770.

Science / Art Area

Žemės ūkio mokslai / Agricultural sciences (A)

Field of Science

Veterinarija / Veterinary (A002)

Keywords (en)

Abstract (en)

Various microRNAs (miRNAs) present in autologous blood products of canines have not been studied recently. We aimed to elucidate the existence of miRNAs in platelet-rich fibrin (PRF) and the stability of canine autologous blood products under various storage conditions. Total RNAs were isolated from PRF and other autologous blood products following newly adapted protocols used in commercial kits for plasma and tissue samples. Quantitative real-time polymerase chain reaction analysis (qPCR) was used to detect miRNAs in autologous blood products. The miR-16, miR-21, miR-155, and miR-146a were abundant in PRF and other autologous blood products of canines. Furthermore, we found they could maintain stability under protracted freezing temperatures of −30 °C for at least one month. Our findings revealed that PRF might be a stable resource for various canine miRNAs.