Experimental investigation of immunostimulating effect of Propolis ethanolic extract

Abstract

Aim: The aim of this experimental investigation was to reveal immunostimulating effect of an ethanolic extract derived from Lithuanian Propolis on cell-mediated immunity. Methods: Material of investigation - the 10% ethanolic extract from Propolis was provided by Lithuanian manufactuter “Medicata Filia”. The immunostimulating potency of Propolis preparation was experimentally investigated using laboratory mice and analysis of certain immunological and clinical laboratory techniques. The amount of leucocytes, thymocytes and splenocytes (109/ l) was counted by laboratory analysis. Blood smears had been made, it was fixed and coloured using Giemsa method. Forms of leucocytes in the leukogram were counted by using immersion system. Results: The investigation was based on evaluation of immunostimulating properties of Lithuanian Propolis extract. The usage of the preparation produced from Lithuanian Propolis has increased general amount of leucocytes and lymphocytes, thymocytes and splenocytes, as immunocompetent cells in experimental mice blood. The results showed, that usage of the Propolis extract increased general amount of leucocytes, especially stimulated the increase of lymphocytes (p<0.05). Propolis preparation activates T and B lymphocytes production (p<0.05) in central (thymus gland) and peripheral (spleen) immune system organs. Conclusions: The results of the study demonstrate that the ethanolic extract from Lithuanian Propolis activates immune system by affecting immunocompetent cells production. From these particular immuno-pharmacological effects of Lithuanian Propolis,