The Technology of a medicinal syrup with pine buds

Abstract

Introduction: Pine buds products are used as antiseptic, tonic but mostly as expectorant in respiratory system disorders [1]. This medical plant chemical composition consists of many essential oils and antioxidants such as vitamin C and some phenolic compounds which mostly consists of phenolic acids (gallic acid, benzoic acid and etc.) [2]. One of the most known medicinal formulation of pine buds is syrup. It is useful to know the amount of the antioxidants in the pine buds’ syrups to be able to assess the health impact of a product. The aim of this study is to produce a single syrup with the best properties, which contains of ethanolic liquid extracts of pine buds. Meterial and methods: A pine buds’ syrup was made and tested in the laboratory scale with three different ethanol extracts of pine buds (with 30%, 35% and 40% of ethanol solutions). The total amount of phenolic compounds within pine buds syrup was measured using spectrophotometric determination on gallic acid. Antioxidant activity was determined spectrophotometrically by DPPH radical scavenging method. Dynamic viscosity and stability studies were conducted. The results were obtained using Microsoft Office Excel 2013 and GraphPadPrism 8.1.0 programs. Results: The lowest amount of phenolic compunds (0,39 mg/ml) and antioxidant properties (15,81%) contained a syrup with the 30% of the ethanol pine buds’ extract. The highest amount of phenolic compounds (0,55 mg/ml) and antioxidant properties (17,74%) contained a syrup with 40% of the ethanol pine buds’ extract. The highest dynamic viscosity (59,61 mP·a) also showed up in a syrup with 40% of the ethanol extract. Stability studies of this particular syrup has shown statistically insignificant differences during the relevant study period. Conclusion: All obtained data between the different syrups was statistically significant. According to the analytical methods, the maximum total amount of phenolic compounds and antioxidant activity were in t