Human induced pluripotent stem cell derived astrocyte based models in neuropsychiatric disorders

Abstract

Objectives 1.Human induced pluripotent stem cell (hIPSC) based model is being developed for research into the pathogenesis of neuropsychiatric disorders and personalized treatment. Materials and Methods First we isolate peripheral blood progenitor cells (PBMCs) from patient‘s, that has a neuropsychiatric disorder, blood. Then, after we run them through culturing process, we start the reprogramming process to create hIPSCs. After that we can start hIPSCs differentiation to astrocyte. Results Astrocytes participate in synaptogenesis, glutamatergic, monoaminergic system and form the blood-brain barrier. Astrocyte dysfunction has been linked to the development of various neuropsychiatric disorders (Schizophrenia, Major depressive disorder and Autism spectrum disorders and Alzheimer’s) by disruption of glutamate homeostasis or it’s signaling pathways. hIPSC models with astrocytes and neurons may help in understanding the pathogenesis of neuropsychiatric disorders and thus aid in drug development. Conclusions Glial cells in general plays a very important role in the nervous system and depending on their functions may be divided into macroglia and microglia;other classes of glial cells have also been identified:oligodendrocyte progenitor cells and specialized glial cells. Glial cells play a major physiological role in the nervous system and it is known that these cells actively parcitipate in the communicational processes of the brain.It is known,that astrocytes, a type of glial cells,actually are actively involved in the processing,transfer and storage of information in the nervous system,which shows, that brain function depends not only on the neuronal network activity, but more so, on the activity of neuron- glia network.Research shows that various malfunctions in glial cells can lead to psychopathology, so these cells may,therefore,serve as appropriate targets for therapeutic interventions. IPSCs could be a[...].