Influence of LG-maize silage on ruminal protozoa and anaerobic microflora of dairy cows
Date |
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2017-09-28 |
ISBN 978-609-473-057-3.
Leidinys išleistas Lietuvos sveikatos mokslų universiteto Veterinarijos akademijos ir Lietuvos Respublikos žemės ūkio ministerijos (projekto sutartis 2017-05-05 Nr. ŠR-17-12/PRM17-39) lėšomis.
The aim of this study was to assess influence of LG-maize silage on ruminal protozoa and anaerobic microflora. For this purpose, 16 clinically healthy crossbreed (lithuanian Black-and-White and Holstein) mid-lactation dairy cows were selected and divided into 2 groups (8 cow/group) applying the principle of analogous. All cows were fed local farm diet, but differed by varieties of maize silage: the control group was fed farm diet with local maize silage, and the experimental group received the farm diet with LG-maize silage. After two weeks adaptation, the experiment lasted for 60 days. At the end of the experiment (d 61) the rumen fluid samples were collected by a stomach tube GDZ-1 (Sederevičius, 2000) 3 hours after morning feeding from three randomly selected cows of each group. The rumen fluid was analysed for protozoa count using Fuchs-Rosenthal counting chamber (blaubrand, Wertheim, Germany) and Olympus microscope (BX43, Hamburg, Germany). The evaluation of total anaerobic bacterial count, lactate fermenting as well as cellulose fermenting bacterial count (gylswyk, 1990) in the rumen fluid of cows were carried. Statistical analysis performed by statistic packet "R 1.7.1." (http://www.rprojekt.org) and WinExel program, the mean values, standard deviation and statistical significance at P≤0.05 estimated. [...].