The Effect of Crataegus extract on heart mitochondrial function
| Author | Affiliation | |
|---|---|---|
Masteiková, Ruta | Department of Pharmaceutics, University of Veterinary and Pharmaceutical Sciences Brno | |
Dvořáčková, Katerina | Departament of Pharmaceutics Faculty of Pharmacy University of Veterinary and Pharmaceutical Sciences, Brno |
| Date |
|---|
2008-10-30 |
This study was aimed to investigate in vitro the influence of different concentrations of Crataegus fruit extract (70 ng/ml – 13.9 μg/ml of phenolic compounds (PC)) on oxidative phosphorylation in isolated rat heart mitochondria. The mitochondrial respiratory rates were determined oxygraphically by means of Clark-type electrode system with different substrates: pyruvate + malate, succinate and palmitoyl-L-carnitine. Our results demonstrate that Crataegus extract at doses under to 278 ng/ml of PC had no effect on mitochondrial functions. At concentrations of 278 ng/ml – 6.95 μg/ml of PC, extract stimulated the State 2 respiration rate by 11–27% with all used substrates. The highest Crataegus extract concentration used in our investigations (13.9 μg/ml of PC) increased the State 2 respiration rate by 27–34% and significantly reduced (12–19%) the maximal ADP-stimulated mitochondrial respiration rate due to the inhibition of mitochondrial respiratory chain.