The influence of the biocatalysators on the formation of higher alkohols and fusel oils during bioethanol production from micronised rye grain

Abstract

The aim of the study was to evaluate the effect of different biocatalysts on the formation on the higher alcohols and fusel oils during bioethanol production from micronised rye grain. The low-temperature technological process was used for bioethanol production under the laboratory conditions. Micronised rye grains were obtained from ecological farm "Ustukiu malunas" (Pasvalys, Lithuania). Simultaneous hydrolysis and saccharification of raw material was performed by using combinations of different biocatalysts: Stargen, Ecopulp TX-200-A, Ceremix, Pentopan MONO BG, Depol 680P and Depol 692L. Baker yeast;s Saccharomyces cerevisiae were used for fermentation. In all cases the alcohol yield and quality were evaluated. The qualitative analysis of alcohol was performed using gas chromatography (GC). The higher alcohols and fusel oils in the end product were quantified using peak areas and known concentrations of corresponding standard compounds. It was found that the largest amount of the higher alcohols and fusel oils (5619.5 mg/l) was obtained using Depol 692L and Stargen preparations for mass saxxharification, and the lowest concentration (2571.2 mg/l) was measured using ...[...].