The Effect of Kaempferol on the Change in Vitality of Breast Cancer Cells in Combination with Ionizing Radiation
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2020-06-26 |
Poster Abstracts.
ISBN 978-609-96167-0-4 (Online).
This project has received funding from the Research Council of Lithuania under Grant Agreement CERN-LSMU-2019-1/PRM19-114.
Background and Objectives Breast cancer is the most common women's oncologic disease and leading cause of morbidity and mortality. The main treatment of breast cancer is radiotherapy, which is based on ionizing radiation (IR) activity. IR causes DNA structure instability and distruption causing activation of DNA repair mechanisms which leads to cell cycle arrest and cell death. Although radiotherapy is widely used and provides substantial benefits it has several limitations. It has been observed, that cancer cells develop radioresistance and are able to repopulate, therefore decreasing effectiveness of the treatment. Consequently, there is a growing interest in compounds which could inhibit cancer cell viability or sensitize them, thus improving treatment efficiency. Phytochemicals have attracted attention of many researchers as potential anti-cancer agent. In addition, phytochemicals might inhance cancer cells sensitivity to IR. Therefore, the aim of this study was to evaluate human breast cancer MCF-7 and MDA-MB-231 cell line response to a single dose of IR in combination with phytochemicals. Material and Method MCF-7 and MDA-MB-231 breast cancer cell lines were used to test kaempferol (KMF) effect. KMF was dissolved in dimethyl sulfoxide. Initially, we examined cell viability after the exposure solely to KMF. In order to assess survival, we incubated cells with different KMF (0, 5, 10, 20, 30, 40 μM) concentrations for various incubation times (24, 48, 72 h). For cells survival analysis MTT test was performed. Afterwards, the combination of KMF and IR (0, 2, 10 Gy) on cells survival (MTT method) was analysed. Results This study showed that KMF had different effect on chosen breast cancer cells survival. There was a significant decline in MCF-7 cell viability which was KMF concentration-dependent. The same effect was observed following all three incubation periods. However, significant decrease of MDA-MB...[...].