Search PDB
- preprintThe New England Journal of Medicine, 2025-03-30, vol. 00, no. 00, p. 1-14
Five risk factors account for approximately 50% of the global burden of cardiovascular disease. How the presence or absence of classic risk factors affects lifetime estimates of cardiovascular disease and death from any cause remains unclear.
- research articleMedicina, 2025-04-02, vol. 61, no. 4, p. 1-13
Background and Objectives: Alcohol consumption has been a longstanding public health concern and known link to violence. The aims of this study were to analyze alcohol-related homicide cases in Lithuania, focusing on the prevalence of binge drinking among perpetrators and victims, the situational and behavioral patterns leading to violence, and the legal outcomes of these cases. Materials and Methods: This study employed a retrospective analysis of court case law of criminal cases of the year 2019. The analysis was conducted by combining qualitative and quantitative analytical approaches. Results: The findings revealed that 84.6% of homicides occurred during binge drinking events. Alcohol intoxication was prevalent among both perpetrators (92.3%) and victims (86.5%), emphasizing the dual role of alcohol in homicide cases. Interpersonal violence was the primary pattern of homicide (78.8%), while planned homicides accounted for 21.2%. Thematic content analysis of the cases revealed that Lithuanian courts consistently regard alcohol consumption as an aggravating factor that contributes to the commission of violent crimes and influences the severity of criminal punishment. This reflects a judicial position that voluntary intoxication does not lessen legal responsibility, despite its effects on impairing judgment, heightening aggression and impulsivity, and escalating conflicts into deadly violence. Conclusions: Our findings revealed that the majority of alcohol-involved homicides occurred during binge drinking events, in a domestic environment, and because of unplanned acts of interpersonal violence. Targeted public health interventions should focus on strengthening alcohol control policies and enforcing stricter regulations to discourage binge drinking environments.
Obesity-driven musculotendinous remodeling impairs tissue resilience to mechanical damagePublication preprintCell and Tissue Research, 2025-03-31, vol. 00, no. 00, p. 1-16Obesity has been associated with lower muscle strength-to-body mass ratio. Here, we evaluated the effects of diet-induced obesity on the mechano-structural properties of isolated muscles and tendons. Thirty 10-week-old male C57BL/6 J mice were randomly assigned to either an obesogenic high-fat diet group (OB) for 24 weeks or a control group (CN) maintained on a standard chow diet. Soleus muscle (SOL) and Achilles tendon (AT) specimens were isolated and subjected either to failure testing, 300 cycles of passive stretch-destretch, or isometric twitch contractions. Morpho-structural and protein expression analyses were conducted to assess collagen and adipose tissue accumulation, concentrations of cross-linking factors, and any alterations in the POSTN-TGFβ1-Akt signaling pathway. OB SOL and AT tissues were more fragile than those from CN (p < 0.05). A piecewise linear regression model revealed a tendency for OB tissues to exhibit steeper mechanical property changes within the first 20 cycles compared to CN, followed by a similar plateau phase in both groups. OB SOL-AT complexes showed a slower twitch-contraction-relaxation pattern than CN (p < 0.05). OB tendons and muscles were larger than those of the CN, with muscles featuring bigger fibers, and higher collagen area fraction (p < 0.05). Elevated TGFβ1 and POSTN concentrations were observed in OB tissues (p < 0.05), alongside increased P-Akt and P-4EBP1 expression (p < 0.05). These findings highlight the detrimental effects of obesity on the structural integrity of muscle and tendon tissues and suggest a significant role of POSTN-TGFβ1-Akt signaling in obesity-associated musculotendinous remodeling.
- research articleGels, 2025-03-26, vol. 11, no. 4, p. 1-21
This study investigates the phytochemical profile, antioxidant and anti-inflammatory properties, and 3D-printing application of Origanum vulgare L. ssp. hirtum extract. The extract revealed a diverse range of phenolic compounds, with rosmarinic acid as the predominant compound (47.76%). The extract showed moderate to high lipoxygenase inhibition (IC50 = 32.0 µg/mL), suggesting its potential as an anti-inflammatory agent. It also exhibited strong antioxidant activity, with hydrogen peroxide scavenging (SC50 = 99.2 µg/mL) and hydroxyl radical scavenging (IC50 = 64.12 µg/mL) capabilities. In cellular studies, high concentrations (50 µg/mL and 100 µg/mL) significantly decreased intracellular ROS production in Caco-2 cells (reductions exceeding 53% and 64%, respectively). Moreover, the extract suppressed NO production in LPS-stimulated J774A.1 macrophages in a concentration-dependent manner. The study also explores the incorporation of the extract into 3D-printed gummies. The gels exhibited a shear-thinning behavior, which was essential for successful extrusion-based 3D printing. The incorporation of Origanum extract significantly influenced the mechanical strength and compaction properties of the 3D-printed gummies before breaking (1.6-fold increase) allowing for a better mouth feeling. PXRD and FTIR analyses confirmed the amorphous nature of the 3D-printed gummies and the interaction between active ingredients and excipients utilized for printing. These findings demonstrated the potential for semisolid extrusion 3D printing at room temperature to transform a culinary herb (Origanum vulgare spp. hirtum) into a healthcare product with antioxidant and anti-inflammatory properties.
- research articleInternational Journal of Molecular Sciences, 2025-03-29, vol. 26, no. 7, p. 1-21
A series of novel hydrazones bearing diphenylamine and 5-oxopyrrolidine moieties, along with benzene and naphthalene rings substituted with hydroxy, alkoxy, or carboxylic groups, were synthesized. Their anticancer activity was evaluated in vitro using both 2D (MTT and ‘wound healing’ assays) and 3D (cell spheroid) models against human melanoma IGR39 cells, the triple-negative breast cancer cell line MDA-MB-231, and pancreatic carcinoma Panc-1 cell line. Compounds 8 (2-hydroxybenzylidene derivative) and 12 (2-hydroxynaphthalenylmethylene derivative) demonstrated the highest cytotoxicity in both 2D and 3D assays, while compounds 4 (2,5-dimethoxybenzylidene derivative) and 6 (2,4,6-trimethoxybenzylidene derivative) were most effective at inhibiting cell migration. Notably, all compounds exhibited lower activity against the Panc-1 cancer cell line in a cell monolayer, but the effects on spheroid cell viability in 3D models were comparable across all tested cancer cell lines. Molecular docking studies of the most active hydrazones suggested that these compounds may act as multikinase inhibitors. In particular, 2-hydroxynaphthalenylmethylene derivative 12 showed high binding affinity values (−11.174 and −11.471 kcal/mol) to the active sites of two key protein kinases—a non-receptor TK (SCR) and STPK (BRAF)—simultaneously.
Lėtinis atrofinis gastritasPublication journal articleLietuvos bendrosios praktikos gydytojas. Kaunas : Vitae Litera, 2008, t. 12, Nr. 10., p. 679-681.Lėtinis atrofinis gastritas – tai ilgalaikio lėtinio skrandžio uždegimo sąlygota skrandžio gleivinės atrofija. Atrofinio gastrito fone vystosi metaplazija žarninio tipo epiteliu, vėliau epitelio displazija ir skrandžio vėžys. Atrofija ir žarninė metaplazija apibūdinamos kaip ikivėžinės būklės. Skrandžio gleivinės atrofija ir žarninė metaplazija yra dažnesnės šalyse, kuriose yra didelis sergamumas skrandžio vėžiu. Atrofinis gastritas laikomas ikivėžine liga, tačiau nėra visiškai aišku, ar galimas atrofijos proceso sustabdymas, ar tai leidžia apsaugoti nuo skrandžio vėžio.
4640 - conference paper7th Japan-Lithuania Joint Life Sciences Symposium: New approach to Life Sciences“ : 18 December, 2020 / Lithuanian University of Health Sciences. Kaunas : Lithuanian University of Health Sciences, 2020., 2020-12-18, p. 1-1
Voice disorders (hoarseness) are a common condition manifesting in almost 10% of the population. The causes of hoarseness can be related to common respiratory diseases and/or to vocal fatigue caused by vocal overload, however it can also be related to functional, behavioral, neurologic factors and both benign and malignant laryngeal tumors. Hoarseness can also be an early symptom of laryngeal cancer. The VoiceScreen app requires the user to perform two simple tasks: 1). Sustain a vowel [a:] sound for 4 seconds, 2) Read the provided sentence. The users’ voice is recorded and then analyzed extracting six acoustic voice function parameters which are then weighted and merged together to provide the user with the Acoustic Voice Quality Index (AVQI) of his/her voice. The value of AVQI as a clinical marker of overall voice quality has already been proven across multiple studies revealing adequate diagnostic accuracy. The AVQI values do not depend on gender and age. The VoiceScreen app calculates and displays the value of AVQI and also provides the user with recommendations based on the test results. The provided recommendations should be taken as guidance on what to do next to help the user’s voice. [...].
3987 Vidutinio trombocitų tūrio sąsajos su lėtiniu prieširdžių virpėjimu ir periferinių arterijų ligaPublication [Mean platelet volume: its relation to permanent atrial fibrillation and peripheral artery disease]research articleMedicinos mokslai. Medical sciences. Kėdainiai : VšĮ Lietuvos sveikatos mokslinių tyrimų centras, 2019, vol. 7, no. 12, Oct 31., 2019-11-06, p. 1-6.Vidutinis trombocitų tūris (MPV-mean platelet volume) yra bendrojo kraujo tyrimo metu nustatomas rodiklis, kuris apibūdina vidutinį trombocitų dydį ir gali būti susijęs su uždegimu bei tromboze. Didesnis MPV yra susijęs su didesne išeminio insulto, ūminio miokardo infarkto, giliųjų venų trombozės bei plaučių arterijų trombembolijos rizika. Tyrimo tikslas - nustatyti MPV sąsajas su lėtiniu prieširdžių virpėjimu (PV) ir periferinių arterijų liga (PAL). Į tyrimą įtraukta 180 pacientų, kurie suskirstyti į grupes: sergančių lėtiniu PV pacientų ir pacientų, kuriems registruotas sinusinis ritmas. Visiems tiriamiesiems apskaičiuotas kulkšnies-žasto indeksas (KŽI), sistolinio arterinio kraujo spaudimo kojose matavimui naudotas 5-MHz dopleris. PAL diagnozuota, kai KŽI gautas <0,9. MPV reikšmės buvo didesnės pacientams sergantiems lėtiniu PV (kontrolinėje grupėje 8,635±1,476 fl, tiriamojoje grupėje 9,131±1,202 fl) (p=0,015). Pacientai, kuriems nustatytas KŽI<0,9 ir MPV>8,55 fl, dažniau sirgo lėtiniu PV nei pacientai, kurių KŽI>0,9, o MPV<8,55 fl (p=0,001). Didesnis MPV buvo susijęs su didesne PAL tikimybe (ŠS 1,608, 95% PI 1,243 – 2,081) (p=0,001) nepriklausomai nuo lyties ir amžiaus (koreguotas ŠS 1,612, 95% PI 1,248 – 2,098) (p=0,001). PAL sergantiems pacientams buvo nustatytas didesnis MPV, kritinė MPV reikšmė - 8,55 fl. MPV reikšmei esant 8,55 fl, rodiklio jautrumas PAL nustatymui buvo 76%, o specifiškumas – 61%.
3293 - journal articleLietuvos bendrosios praktikos gydytojas. Kaunas : Vitae Litera, 2017, t. 21, Nr. 2., 2017-03-13, p. 122-125.
Mezenterinis panikulitas (MP) – reta patologija, apibūdinama kaip lėtinis, nespecifinis žarnyno pasaito riebalinio audinio uždegimas. Ligos paplitimas – 0,16-2,5 proc. Vyrams ši patologija dažnesnė nei moterims, santykis – 2-3:1. Pagrindinis patogeninis mechanizmas yra nespecifinis uždegiminis atsakas į skirtingus etiologiniuis faktorius, tačiau tikslios priežastys iki šiol nepakankamai ištirtos. Histologiškai išskiriamos trys ligos progresavimo stadijos. Dažniausiai ši liga yra besimptomė ir nustatoma atsitiktinai, kitais atvejais simptomai būna nespecifiniai ir kintami. Kompiuterinė tomografija yra pagrindinis tyrimas MP diagnozei nustatyti. Tiksliai diagnozei patvirtinti reikalinga ekscizinė biopsija ir histologinis tyrimas. Nėra visuotinai priimto sutarimo, kokį gydymą skirti, rekomenduojama gydyti imunomoduliatoriais, priešuždegiminiais, antifibrotiniais vaistais. Chirurginis gydymas gali būti taikomas, jei konservatyvusis gydymas neveiksmingas arba yra gyvybei pavojingų komplikacijų.
3104 Žmogaus anatomija : [vadovėlis] T. 2Publication bookKaunas : Lietuvos sveikatos mokslų universiteto Leidybos namai, 2016., 2016-10-02Vadovėlyje "Žmogaus anatomija" aprašoma žmogaus išorinė ir vidinė sandara organų sistemų, vykdančių organizmo kraujo ir limfos apykaitą, imuninę apsaugą, nervinę ir endokrininę reguliaciją, pateikiama organų raidos schema, pabrėžiama anatominių savitumų medicininė reikšmė. Vadovėlis skiriamas biomedicinos krypties medicinos ir odontologijos studijoms, juo galės naudotis ir rezidentai, doktorantai ir kiti specialistai. Vilniaus ir Kauno medicinos anatomai, 2003 m. rekomendavus LR švietimo ir mokslo ministerijos Aukštųjų mokyklų bendrųjų vadovėlių leidybos komisijai, parengė dviejų dalių vadovėlį „Žmogaus anatomija". Antroji jo dalis, parengta Kauno anatomų, padedant Vilniaus anatomei prof. Janinai Tutkuvienei, buvo išleista 2007 m., o pataisyta 2-oji laida - 2008 m. Šioje dalyje pateiktos anatomijos žinios apie organų sistemas, vykdančias organizmo kraujo ir limfos apykaitą, imuninę apsaugą, nervinę ir endokrininę reguliaciją, apie jutimo organus ir odą. Į šio vadovėlio autorių sąrašą taip pat buvo įtrauktas vokiečių anatomas Gertas-Horstas Šumacheris, leidęs vartoti kai kurias jo anatomijos vadovėlio iliustracijas.Sudėvėti vadovėlio egzemplioriai skaityklose ir jų dingimas knygynų lentynose rodo, kad jis turėjo paklausą, buvo naudingas siekiantiems anatomijos žinių žmonėms. Autoriams malonu tai žinoti ir esame dėkingi LSMULeidybos namams, sumaniusiems išleisti dar vieną jo laidą. Tad atsirado gera proga šioje laidoje, iš esmės nekeičiant vadovėlio dalykinio turinio, atlikti kai kuriuos pataisymus, papildymus ir jo sandaros pakeitimus. Pirmiausia buvo ištaisytos pačių autorių ir skaitytojų pastebėtos techninės ir dalykinės klaidos, koreguoti kai kurie lietuviški anatomijos terminai, atnaujinta bei išplėsta lietuviškų ir lotyniškų terminų rodyklė. Kai kurie skyriai papildyti aprašomų organų ontogenezės,
3062 226
Kardiologijos pagrindai studentamsPublication bookKaunas :: Lietuvos sveikatos mokslų universitetas,, 2020., 2020-04-28, p. 1-265„Kardiologijos pagrindai studentams “ apima širdies ir kraujagyslių ligų sindrominę diagnostiką, paciento ištyrimo metodiką, kardiologijoje vartojamų vaistų klinikinę farmakologiją, vaizdinius tyrimo metodus, taip pat atskirų širdies ir kraujagyslių ligų diagnostiką bei gydymą. Jis skirtas medicinos specialybės studentų, taip pat kardiologijos ir kitų vidaus ligų specialybių gydytojų rezidentų mokymui, vidaus ligų, skubiosios pagalbos gydytojų, gydytojų kardiologų bei kitų specialybių gydytojų kvalifikacijos kėlimui.
1072 2793 - bookKaunas : Lietuvos sveikatos mokslų universiteto Akademinė leidyba, 2021, 2021-09-22, p. 1-104
Mokomoji knyga skirta pirmojo kurso Veterinarinės medicinos studentams, studijuojantiems „Atramos ir judėjimo“ modulį. Osteologija – mokslas apie kaulus – sudaro didelę šio modulio dalį. Šia mokomąja knyga galės naudotis ir studentai, studijuojantys modulio „Gyvybės mokslai – 1“ bendrosios anatomijos dalį. Knyga taip pat bus naudinga aukštesniojo kurso studentams, studijuojantiems chirurgijos ir ortopedijos studijų dalykus, žemės ūkio srities magistrantams bei doktorantams, veterinarijos gydytojams. Veterinarinės anatomijos literatūros studijoms lietuvių kalba labai trūksta, o vos keli išleisti vadovėliai mena praeitą šimtmetį. Manome, kad šis leidinys atitiks šiuolaikinės veterinarinės medicinos poreikius savo turiniu, struktūra bei iliustracijomis ir padės studentams geriau įsisavinti veterinarinės osteologijos žinias. Šiame naujame leidinyje išsamiai apžvelgta mėsėdžių griaučių struktūra, kuri iki šiol nebuvo labai akcentuojama, tačiau laikui bėgant tapo viena aktualiausių ir labiausiai tiriamų sričių veterinarinėje praktikoje. Studentams osteologija yra vienas pirmųjų prisilietimų prie veterinarinės anatomijos mokslo, todėl svarbu užtikrinti, kad šis įspūdis neišblėstų ir motyvuotų toliau semtis anatomijos žinių. Knygoje pateikiama išsami ašinių ir pridėtinių griaučių anatominė sandara, nagrinėjami visų aktualiausių naminių gyvūnų – atrajotojų (galvijų, avių, ožkų), arklių, kiaulių, mėsėdžių (šunų ir kačių) rūšiniai skirtumai. Knyga gausiai iliustruota geros kokybės nuotraukomis, kurių didžioji dalis yra nufotografuota pačių autorių. Pateikiami anatomijos terminai (lietuvių ir lotynų kalbomis) suderinti su „Veterinarinės anatomijos, histologijos ir embriologijos terminų žodynu“ (2013).
442 485 - doctoral thesisKaunas,:, 2014-07-04
[...]. Tyrimo tikslas. Nustatyti neurosonografinių galvos ir kaklo arterijų parametrų vertę prognozuojant demenciją lengvą kognityvinį sutrikimą turintiems pacientams. Tyrimo uždaviniai. 1. Ištirti lengvą kognityvinį sutrikimą turinčių pacientų demografines ir klinikines charakteristikas ir įvertinti jų ir progresavimo į demenciją ryšį. 2. Ištirti lengvą kognityvinį sutrikimą turinčių pacientų ekstrakranijinės kraujotakos parametrus ir įvertinti jų ir progresavimo į demenciją ryšį. 3. Ištirti lengvą kognityvinį sutrikimą turinčių pacientų intrakranijinės kraujotakos parametrus ir įvertinti jų ir progresavimo į demenciją ryšį. 4. Ištirti lengvą kognityvinį sutrikimą turinčių pacientų miego arterijų sienelių parametrus ir įvertinti jų ir progresavimo į demenciją ryšį. 5. Ištirti lengvą kognityvinį sutrikimą turinčių pacientų miego arterijų aterosklerozinius požymius ir įvertinti jų ir progresavimo į demenciją ryšį. 6. Įvertinti bendrą tiriamųjų demografinių ir klinikinių charakteristikų bei neurosonografinių parametrų diagnostinę reikšmę prognozuojant progresavimą iš lengvo kognityvinio sutrikimo į demenciją. [...].
456 144 - bookKaunas :: Kauno medicinos universitetas,, 2008.
178 456 - doctoral thesisKaunas,:, 2008-03-07
377 120
- journal articleDiabetologia. Berlin : Springer, 2007, vol. 50, no. 12., 2007-08-07, p. 2439-2442.
Aims/hypothesis The aims of this study were to provide a contemporary picture of mortality and causes of death in Europe following a diagnosis of type 1 diabetes made before the 15th birthday, and to examine excess mortality by country for possible links to incidence level or national prosperity. Methods Thirteen population-based EURODIAB registers in 12 countries followed-up 28,887 children diagnosed since 1989, either by record linkage to population registers or through contact with doctors providing care. Results There were 141 deaths in the cohort during 219,061 person-years of follow-up compared with 69.1 deaths expected from national mortality rates, a standardised mortality ratio (SMR) of 2.0 (95% CI 1.7–2.4). The SMR varied from 0 to 4.7 between countries, but showed little relationship with the country’s incidence rate or gross domestic product (US$ per capita). The SMR did not change significantly with attained age, calendar period or time since diagnosis. The female SMR (2.7; 95% CI 2.0–3.5) was greater than the male SMR (1.8; 95% CI 1.4–2.2), although absolute numbers of excess deaths were similar in the two sexes. One-third of deaths were classified as directly attributable to diabetes (many with mention of ketoacidosis) and half were unrelated to diabetes. There was a non-significant excess of accidental/violent deaths (48 observed vs 40.7 expected; SMR 1.2; 95% CI 0.9–1.6) but little excess in suicides (11 observed, 10.2 expected; SMR 1.1; 95% CI 0.5–1.9). Conclusions/interpretation Before the onset of late complications, significant excess mortality existed following the diagnosis of type 1 diabetes in childhood, even in recent years. Variation between countries in this excess could not be explained. Electronic supplementary material The online version of this article (doi:10.1007/s00125-007-0824-8) contains supplementary material, which is available to authorised users.
5 - conference paperУcпexи гeпaтoлoгии. Pига, 1971., p. 292-310.
5 - conference paper63rd International conference for students of physics and natural sciences Open Readings 2020 : 17-20 March 2020, Vilnius, Lithuania : abstract book / Faculty of Physics. Vilnius University. Vilnius : Vilnius University, 2020. ISBN 9786090703779., 2020-03-17, p. 507-507
Chemical analysis of spice, aromatic plants at different vegetation stages can serve for the optimal collection of plant raw material revealing the period when the plant possesses the highest biological activity and accumulates the highest content of biologically active compounds, which could be used in pharmacy, medicine, food or cosmetic industry [1]. The aim of this study was to investigate the qualitative and quantitative composition of essential oils obtained from A. absinthium during different vegetation stages. The object of investigation was Artemisia absinthium L. a perennial medicinal, aromatic plant of Asteraceae (Bercht. & J. Presl) family. A. absinthium is widely used in the Lithuanian folk medicine mainly for stomachic and anthelmintic activity, also for healing gall-bladder and kidneys diseases, against insomnia, diarrhea [3, 5, 6, 7]. Raw material of A. absinthium was collected during different vegetation stages of vegetation cycle in Spice − Melliferous plants collection ex situ of Botanical Garden at Vytautas Magnus University in 2018. Five stages have been separated: growth and leaf production, flower bud development, beginning of the flowering, massive flowering, end of the flowering [4]. The essential oils have been extracted by hydrodistillation method and analysed by the chromatographic techniques in the gas phase and chromatography in gas phase coupled with mass spectrometry (GC/MS) so as to determine their chemical composition. The percentage composition of the essential oils was computed from GC peak areas without correction factors. Qualitative analysis was based on a comparison of retention times, indexes and mass spectra with the corresponding data in the literature [2] and computer mass spectra libraries. There were studied 5 samples of A. absinthium considering different vegetation stages. A total of 81 different compounds were found in the essential oils. The data showed the...[...].
7 A Synergistic activity of Hsp90 inhibitors and anticancer drugs in pancreatic cancer cell linesPublication conference paperVita Scientia : International conference „Vita Scientia“ : conference book : 3rd January, 2018, Vilnius / Vilnius University. Vilnius : Vita est Scientia, 2018, 2018., 2018-01-03, p. 53-54Heat shock protein 90 (Hsp90) is a molecular chaperone with over 200 identified client proteins. This chaperone protein is crucial for protein folding and stabillization and interfere in many pathways of cancer cells, therefore it is a potential target for anticancer therapy. Hsp90 inhibitors bind to the N-terminal ATP domain of Hsp90, and several of them have entered clinical trials. Recent studies show that Hsp90 inhibitors in combination with clinically used chemotherapeutic agents may act synergistically, therefore such effect can reduce the dose and toxicity of used drugs, and minimize or delay the induction of drug resistance [1]. The aim of our study was to evaluate activity of different combinations of two Hsp90 inhibitors (ICPD47 and ICPD62) and three anticancer agents (gemcitabine, 5fluorouracil and doxorubicin) against pancreatic cancer cells. The effect of Hsp90 inhibitors, other agents and different combinations on cell viability was evaluated by MTT assay in two pancreatic cancer cell lines: MIA PaCa-2 and Panc-1. The type of combination effect was determined by calculating combination index (CI), using CompuSyn software based on Chou-Talalay method [2]. Synergism was considered when CI < 1, antagonism when CI > 1, and CI value of 1 defined an additive effect of the drug combination. Results. Among tested Hsp90 inhibitors ICPD62 had the greatest effect on cell viability (EC50 values after 72 h on MIA PaCa-2 and Panc-1 were 0.446 ± 0.06 µM and 0.41 ± 0.08 µM, respectively) and among other anticancer agents doxorubicin showed the greatest effect on cell viability (EC50 values after 72 h on MIA PaCa-2 and Panc-1 were 0.076 ± 0.03 µM and 0.074 ± 0.02 µM, respectively). The combination of ICPD47 and gemcitabine with concentration ratio 5:1, showed the greatest synergistic activity in MIA PaCa-2 cell line (CI = 0.192, when fa = 0.5), and the combination of ICPD62 and gemcitabine with concentration rat[...].
10 Математические методы и вычислительная техника в теории и практике электрокардиологииPublication [Mathematical methods and computers in theoretical and practical electrocardiology.]research articleТеория и практика автоматизации в кардиологии : [тематический сборник научных трудов ИБС] / [Редкол.: З.И. Янушкевичус (отв. ред.) и др.] ; Министерство высшего и среднего специального образования Литовской ССР. Научно-исследовательский институт физиологии и патологии сердечно-сосудистой системы при Каунасском медицинском институте. Вильнюс : Редакционно-издательский совет М-во высш. и сред. спец. образования ЛитССР, 1980., p. 3-11.Распространение сердечно-сосудистых заболеваний и расширение объема борьбы с ними обусловило значительное увеличение масштабов обследования населения. Обработка этих данных, обязательно включающих электрокардиологические показатели, уже невозможна без применения математических методов и вычислительной техники. Первые работы по применению ЭВМ для анализа электрокардиограмм (ЭКГ) появились в конце пятидесятых годов, а в начале шестидесятых – первые экспериментальные системы анализа ЭКГ, промышленный выпуск которых начался в конце шестидесятых годов в США. По данным Кейсерс, только в США в 1970 году около 50 фирм м компаний занимались разработкой и распространением систем компьютерного анализа ЭКГ. По данным Rautaharju, в настоящее время в капиталистических странах действуют по крайней мере 20 больших центров комьютерного анализа ЭКГ, которые в 1976 году проанализировали около 5 миллионов ЭКГ, в том числе фирмы IBM и Telemed по 1 миллиону 400 тысяч ЭКГ, фирма Hewlett-Packard – 600.000 ЭКГ. [...].
5
- journal articleDiabetologia. Berlin : Springer, 2007, vol. 50, no. 12., 2007-08-07, p. 2439-2442.
Aims/hypothesis The aims of this study were to provide a contemporary picture of mortality and causes of death in Europe following a diagnosis of type 1 diabetes made before the 15th birthday, and to examine excess mortality by country for possible links to incidence level or national prosperity. Methods Thirteen population-based EURODIAB registers in 12 countries followed-up 28,887 children diagnosed since 1989, either by record linkage to population registers or through contact with doctors providing care. Results There were 141 deaths in the cohort during 219,061 person-years of follow-up compared with 69.1 deaths expected from national mortality rates, a standardised mortality ratio (SMR) of 2.0 (95% CI 1.7–2.4). The SMR varied from 0 to 4.7 between countries, but showed little relationship with the country’s incidence rate or gross domestic product (US$ per capita). The SMR did not change significantly with attained age, calendar period or time since diagnosis. The female SMR (2.7; 95% CI 2.0–3.5) was greater than the male SMR (1.8; 95% CI 1.4–2.2), although absolute numbers of excess deaths were similar in the two sexes. One-third of deaths were classified as directly attributable to diabetes (many with mention of ketoacidosis) and half were unrelated to diabetes. There was a non-significant excess of accidental/violent deaths (48 observed vs 40.7 expected; SMR 1.2; 95% CI 0.9–1.6) but little excess in suicides (11 observed, 10.2 expected; SMR 1.1; 95% CI 0.5–1.9). Conclusions/interpretation Before the onset of late complications, significant excess mortality existed following the diagnosis of type 1 diabetes in childhood, even in recent years. Variation between countries in this excess could not be explained. Electronic supplementary material The online version of this article (doi:10.1007/s00125-007-0824-8) contains supplementary material, which is available to authorised users.
5 - conference paperУcпexи гeпaтoлoгии. Pига, 1971., p. 292-310.
5 - conference paper63rd International conference for students of physics and natural sciences Open Readings 2020 : 17-20 March 2020, Vilnius, Lithuania : abstract book / Faculty of Physics. Vilnius University. Vilnius : Vilnius University, 2020. ISBN 9786090703779., 2020-03-17, p. 507-507
Chemical analysis of spice, aromatic plants at different vegetation stages can serve for the optimal collection of plant raw material revealing the period when the plant possesses the highest biological activity and accumulates the highest content of biologically active compounds, which could be used in pharmacy, medicine, food or cosmetic industry [1]. The aim of this study was to investigate the qualitative and quantitative composition of essential oils obtained from A. absinthium during different vegetation stages. The object of investigation was Artemisia absinthium L. a perennial medicinal, aromatic plant of Asteraceae (Bercht. & J. Presl) family. A. absinthium is widely used in the Lithuanian folk medicine mainly for stomachic and anthelmintic activity, also for healing gall-bladder and kidneys diseases, against insomnia, diarrhea [3, 5, 6, 7]. Raw material of A. absinthium was collected during different vegetation stages of vegetation cycle in Spice − Melliferous plants collection ex situ of Botanical Garden at Vytautas Magnus University in 2018. Five stages have been separated: growth and leaf production, flower bud development, beginning of the flowering, massive flowering, end of the flowering [4]. The essential oils have been extracted by hydrodistillation method and analysed by the chromatographic techniques in the gas phase and chromatography in gas phase coupled with mass spectrometry (GC/MS) so as to determine their chemical composition. The percentage composition of the essential oils was computed from GC peak areas without correction factors. Qualitative analysis was based on a comparison of retention times, indexes and mass spectra with the corresponding data in the literature [2] and computer mass spectra libraries. There were studied 5 samples of A. absinthium considering different vegetation stages. A total of 81 different compounds were found in the essential oils. The data showed the...[...].
7 A Synergistic activity of Hsp90 inhibitors and anticancer drugs in pancreatic cancer cell linesPublication conference paperVita Scientia : International conference „Vita Scientia“ : conference book : 3rd January, 2018, Vilnius / Vilnius University. Vilnius : Vita est Scientia, 2018, 2018., 2018-01-03, p. 53-54Heat shock protein 90 (Hsp90) is a molecular chaperone with over 200 identified client proteins. This chaperone protein is crucial for protein folding and stabillization and interfere in many pathways of cancer cells, therefore it is a potential target for anticancer therapy. Hsp90 inhibitors bind to the N-terminal ATP domain of Hsp90, and several of them have entered clinical trials. Recent studies show that Hsp90 inhibitors in combination with clinically used chemotherapeutic agents may act synergistically, therefore such effect can reduce the dose and toxicity of used drugs, and minimize or delay the induction of drug resistance [1]. The aim of our study was to evaluate activity of different combinations of two Hsp90 inhibitors (ICPD47 and ICPD62) and three anticancer agents (gemcitabine, 5fluorouracil and doxorubicin) against pancreatic cancer cells. The effect of Hsp90 inhibitors, other agents and different combinations on cell viability was evaluated by MTT assay in two pancreatic cancer cell lines: MIA PaCa-2 and Panc-1. The type of combination effect was determined by calculating combination index (CI), using CompuSyn software based on Chou-Talalay method [2]. Synergism was considered when CI < 1, antagonism when CI > 1, and CI value of 1 defined an additive effect of the drug combination. Results. Among tested Hsp90 inhibitors ICPD62 had the greatest effect on cell viability (EC50 values after 72 h on MIA PaCa-2 and Panc-1 were 0.446 ± 0.06 µM and 0.41 ± 0.08 µM, respectively) and among other anticancer agents doxorubicin showed the greatest effect on cell viability (EC50 values after 72 h on MIA PaCa-2 and Panc-1 were 0.076 ± 0.03 µM and 0.074 ± 0.02 µM, respectively). The combination of ICPD47 and gemcitabine with concentration ratio 5:1, showed the greatest synergistic activity in MIA PaCa-2 cell line (CI = 0.192, when fa = 0.5), and the combination of ICPD62 and gemcitabine with concentration rat[...].
10 Математические методы и вычислительная техника в теории и практике электрокардиологииPublication [Mathematical methods and computers in theoretical and practical electrocardiology.]research articleТеория и практика автоматизации в кардиологии : [тематический сборник научных трудов ИБС] / [Редкол.: З.И. Янушкевичус (отв. ред.) и др.] ; Министерство высшего и среднего специального образования Литовской ССР. Научно-исследовательский институт физиологии и патологии сердечно-сосудистой системы при Каунасском медицинском институте. Вильнюс : Редакционно-издательский совет М-во высш. и сред. спец. образования ЛитССР, 1980., p. 3-11.Распространение сердечно-сосудистых заболеваний и расширение объема борьбы с ними обусловило значительное увеличение масштабов обследования населения. Обработка этих данных, обязательно включающих электрокардиологические показатели, уже невозможна без применения математических методов и вычислительной техники. Первые работы по применению ЭВМ для анализа электрокардиограмм (ЭКГ) появились в конце пятидесятых годов, а в начале шестидесятых – первые экспериментальные системы анализа ЭКГ, промышленный выпуск которых начался в конце шестидесятых годов в США. По данным Кейсерс, только в США в 1970 году около 50 фирм м компаний занимались разработкой и распространением систем компьютерного анализа ЭКГ. По данным Rautaharju, в настоящее время в капиталистических странах действуют по крайней мере 20 больших центров комьютерного анализа ЭКГ, которые в 1976 году проанализировали около 5 миллионов ЭКГ, в том числе фирмы IBM и Telemed по 1 миллиону 400 тысяч ЭКГ, фирма Hewlett-Packard – 600.000 ЭКГ. [...].
5