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Antimicrobial susceptibility of Escherichia coli isolated from small animals in Lithuania / M. Ruzauskas, M. Virgailis, R. Siugzdiniene, I. Klimiene, L. Vaskeviciute, S. Ramonaite, J. Zymantiene, R. Mockeliunas
Type of publication
Konferencijų tezės nerecenzuojamame leidinyje / Conference theses in non-peer-reviewed publication (T2)
Author(s)
Maisto saugos ir kokybės katedra (U630600) | |
Title
Antimicrobial susceptibility of Escherichia coli isolated from small animals in Lithuania / M. Ruzauskas, M. Virgailis, R. Siugzdiniene, I. Klimiene, L. Vaskeviciute, S. Ramonaite, J. Zymantiene, R. Mockeliunas
Publisher (trusted)
Complutense University of Madrid |
Date Issued
Date Issued |
---|
2014-10-01 |
Extent
p. 443-443.
Is part of
III International Conference on Antimicrobial Research - ICAR2014 : Madrid (Spain), 1-3 October 2014 : Book of Abstracts / Complutense University of Madrid ; Committee: A. Méndez-Vilas, J.A. Mesa González, A. Solano Martín, [et al.]. Madrid : Complutense University of Madrid, 2013.
Version
Originalus / Original
Series/Report no.
Session: Antimicrobial resistance. Mechanisms of action of antimicrobial agents.
Description
Bibliogr.: p. 443
Field of Science
Abstract
Escherichia coli is a commensal organism in people and animals but is also a causative agent of diarrhoea and extra-intestinal infections [1]. The sepsis-associated human mortalities due to E. coli are estimated at 868,000 per year globally [2]. Small animals often are more resistant to E. coli infections however, from public health perspective this species may represent a reservoir of virulence and resistance genes [1]. The aim of this study was to isolate E. coli from small animals and to determine susceptibility to antimicrobial classes important for humans. Rectal (n=200), vaginal (n=100) as well as other (n=80) samples from different organs (skin, eyes, throat) were taken from 380 animals (272 dogs, 55 cats and 53 other species) on small animal clinics in Lithuania. Both diseased (n=280) and healthy (n=100) animals were included in this study. Clinical material was inoculated onto TBX Agar (Biolife, Italy). Single suspected colony of E. coli from one sample was randomly selected for testing. Bacterial identification was performed using Microgen GNA+B identification system. The initial susceptibility testing was performed by disk-diffusion method. Resistant isolates to at least two antimicrobials were further tested for Minimal inhibitory concentrations (MIC’s) using SENSITITRE (Thermo Scientific) plates and ARIX 2X system. Results were interpreted according to EUCAST clinical breakpoints. PCR was used for detection of genes encoding antimicrobial resistance. Two hundred and sixty isolates of E. coli were obtained (68%) from the animals tested. The rate of isolated E. coli from rectum was 90%, from vaginal samples - 50% and 38% from the other organs. The most common resistances were demonstrated to ampicillin (39%), sulfamethoxazole/trimethoprim (16%) and ciprofloxacin (10%). Resistance to gentamicin was detected in 5% of the isolates. Extended spectrum beta-lactamases were produced by 3% of the isolates with attribution to the TEM gene. The only one i
Type of document
type::text::conference output::conference proceedings::conference paper
Other Identifier(s)
(LSMU ALMA)990000850860107106
Coverage Spatial
Ispanija / Spain (ES)
Language
Anglų / English (en)
Affiliation(s)