Use this url to cite publication: https://hdl.handle.net/20.500.12512/91822
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Antimicrobial resistance in E. coli isolates from dogs in Lithuania / Ruzauskas M., Siugzdiniene R., Klimiene I., Virgailis M., Zymantiene J., Vaskeviciute L., Dauksiene A., Mockeliunas R
Type of publication
Konferencijų tezės nerecenzuojamame leidinyje / Conference theses in non-peer-reviewed publication (T2)
Author(s)
Title
Antimicrobial resistance in E. coli isolates from dogs in Lithuania / Ruzauskas M., Siugzdiniene R., Klimiene I., Virgailis M., Zymantiene J., Vaskeviciute L., Dauksiene A., Mockeliunas R
Publisher (trusted)
European Society of Clinical microbiology and Infectious Diseases
Date Issued
2014-05-10
Extent
1 skelb., no. R083.
Is part of
24th European Congress of Clinical Microbiology and Infectious Diseases (ECCMID 2014) [elektroninis išteklius] : Barcelona, Spain, 10–13 May 2014 / European Society of Clinical microbiology and Infectious Diseases – ESCMID ; Committee: Jordi Vila Estape, Winfried V. Kern. Barcelona : ESCMID, 2014.
Version
Originalus / Original
Series/Report no.
Poster Session.
Poster Session.
Field of Science
Abstract
Objectives: To investigate antimicrobial resistance of E. coli isolated from dogs in Lithuania. Methods: Between April 2012 and December 2013 rectal samples using sterile cotton swabs were taken from 250 dogs on small animal clinics in Lithuania. The animals were randomly selected for testing irrespective of their clinical conditions. Clinical material was delivered during 1 hour and inoculated onto MacConkey Agar and Eosin-Methylene Blue (Levine) Agar (Thermo Scientific). Bacterial identification was performed using Microgen GNA+B identification system and manufacturers software (Microgen ID). The susceptibility testing was performed by disk-diffusion method followed by MICs determination using SENSITITRE (Thermo Scientific) plates. Results were interpreted according to EUCAST clinical breakpoints. PCR was used for the detection of genes encoding antimicrobial resistance. Results: One hundred and eighty nine isolates of E. coli were obtained from faeces of tested dogs (75,6%). The most common resistance was demonstrated to ampicillin (39,4%), attributed to TEM gene whereas SHV gene was not detected. Resistance to other beta-lactams was low however, 3,2% of the isolates were associated with ampC type of resistance, while CTX-M genes were detected in 2,1% of the isolates. Resistance to co-trimoxazole was found in 16,4% of the isolates, attributed to genes encoding resistance both to sulfonamides (sul1, sul2 and sul3) and trimethoprim (dfr1, dfr5 and dfrA7). 5,8% of the isolates were resistant to gentamicin and harboured aac(3)II, aphA1 and/or aadA genes. Resistance to ciprofloxacin was detected in 10,4% of the isolates. Resistance to chloramphenicol detected in a single isolates (3,2%), attributed to cml gene. Conclusions: The situation on antimicrobial resistance in E. coli spread in Lithuanian population of dogs is still favourable however, ESBL producing isolates are found in rare occasions. Resistance to fluoroqu
Type of document
type::text::conference output::conference proceedings::conference paper
Other Identifier(s)
(LSMU ALMA)990000844290107106
Coverage Spatial
Ispanija / Spain (ES)
Language
Anglų / English (en)