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Neurochemistry of the rabbit atrial ganglia
Date Issued |
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2018-07-05 |
ISBN 978-80-88214-11-3.
Background: Several types of cardiac arrhythmias are considered to be caused by a disoerdered intrinsic cardiac nerve system (INS). The rabit is widely used in laboratory experiments aimed to improve the treatment of neurogenic arrhythmia. Conversely, the INS in the rabbit atria is morphologically determined employing histochemical staining for acetylcholinesterase and there are very few data on neurochemistry of intrinsic ganglia. Therefore, the aim of the present study was to identify the neurochemical properties of ganglionic cells from the rabbit atria. Material and methods: 12 juvenile rabbit (aged 4-6 weeks) hearts were used in this study. Double immunohistochemical labeling against choline acetyltransferase (ChAT), tyrosine hydroxylase (TH), neuronal nitric oxide synthase (nNOS), substance P (SP), calcitonin gene-related peptide (CGRP) was used employing both fluorescent and laser scanning microscopes. [...].