Modification of cell cultures and functional studies
Cells are transfected with siRNAs or miRNAs of the customer’s choice, and functional assays are performed in cell cultures (viability assays, colony formation, migration assays, etc.).
In vitro cytotoxicity assessment
Toxicity assessment of chemicals, biologicals and their preparations in cell culture. Parameters to be determined include: cellular metabolic activity, viability, death pathway, activation of the inflammatory response, oxidative stress, mitochondrial and glycolytic energy activity.
In vitro evaluation of anti-inflammatory effects
Evaluate the effect (efficacy) of agents and medical devices on viral (including COVID-19) or bacterial inflammation in vitro in a selected tissue model. Determine the production and secretion of immune and/or other markers of immunometabolic activity of selected cells induced by viral or bacterial agents, such as production and secretion of pro-inflammatory cytokines and chemokines, production of metalloproteinases, production of reactive oxygen and nitrogen species, mitochondrial-glycolytic energy metabolism transition, etc. Possible models include the respiratory tract, blood vessel, myocardium, gastrointestinal tract, etc.
Efficacy studies in an in vitro model of myocardial ischemia
Determine whether the investigational agent protects cardiomyocytes from ischemic death. The study may be extended to assess the effects on different death pathways (apoptosis, necrosis, ferroptosis, etc.), functionality (contractility) and mitochondrial damage.
Efficacy studies in an in vitro model of cerebral ischemia
Determine whether the substance being tested protects neurons from ischemia-induced death. Optional assessment parameters include neuronal death rate, number of synapses, synaptic activity and signal quality, level of inflammatory activity of astrocytes and microglia, mitochondrial and glycolytic functionality, and level of inflammatory factors.
Drug efficacy studies in an in vitro model of neurodegeneration
It assesses how the substance protects neurons (cultured together with other brain cells such as microglia and astrocytes) from neurotoxicity. Optional assessment parameters include neuronal death rate, number of synapses, synapse activity and signal quality, level of inflammatory activity of astrocytes and microglia.
Studies on effects on mitochondrial function
The effect of the product on the efficiency of the mitochondrial respiratory chain and phosphorylation system, the integrity of the inner and outer membranes, and other mitochondrial health parameters of the selected tissue is assessed.
Studies on effects on glycolytic activity
The effect of the product on the glycolytic efficiency of the selected tissue and the capacity of the glycolytic system is assessed.
Investigating the elicitation of an inflammatory response in human immune cells
Assess whether the test substances induce inflammation in immune cells such as macrophages, microglia, fibroblasts, astrocytes etc. The level of inflammation shall be assessed by the production and secretion of cytokines and chemokines, the production of metalloproteinases, the production of active oxygen and nitrogen compounds, the mitochondrial-glycolytic energy metabolism transition, etc.
Studies on anticancer effects
The effects of chemicals, biologicals and drugs in cell monolayers and three-dimensional (3D) cultures are investigated. Effects on viability, proliferation, migration, oncogenic factor production intensity and other parameters are determined.
In vitro/ex vivo multi-barrier permeation studies
It assesses the permeation of substances across various barriers: blood-brain, intestinal, vascular endothelial, skin, respiratory epithelial and other barriers in vitro.
Regenerative impact assessment
The regenerative effect on the cells of the selected tissue is evaluated using methods such as wound healing, total metabolic activity, energy metabolism intensity and others. Available models include skin (epidermis, mesoderm, hypodermis, full thickness), respiratory tract, brain, intestine, myocardium, skeletal muscle, retinal neuroepithelium, stem cells, immune cells, etc.
Evaluation of the effects on skin maintenance and functionality in a 3D in vitro model
The effect of the test substance on skin structure (layer thickness and shape, formation of characteristic layers) and function (production of extracellular filler proteins) is investigated in a 3D model of skin cultured in the air-liquid phase boundary.
Assessment of the maintenance of brain functionality in 3D in vitro models replicating natural cerebellar structure and functionality
Investigating neuronal viability and functionality by replicating the cellular composition, structure and function of the natural brain in 3D in vitro models.
Assessment of effects on osteogenesis
Effects on osteoblast viability, proliferation and differentiation are assessed.
Evaluation of the effects of substances in an in vitro model of osteonecrosis
The effect of the test substances on the viability, proliferation and energy metabolism of osteoblasts inhibited by bisphosphonates.
In vitro studies of the effects of neurodegenerative preparations in a model of Alzheimer’s disease
The models can be sporadic (based on amyloidogenic peptides), familial (based on mutation) or mixed. Determine whether the investigational agent protects neuronal synapses, improves their function, reduces inflammation and improves energy metabolism.
Ex vivo evaluation of the effects of medicinal substances or devices in the vasculature
Determine how the chosen drug or device affects the blood vessels ex vivo.
Ex vivo effects of ex vivo drug treatment on blood vessels by ultrasound modulation
Determine how the chosen drug or device affects the blood vessels ex vivo.
Isolation of lipocytes from lipoaspirate and assessment of their viability and determination of hemoglobin in lipoaspirate
Lipocyte viability/metabolic activity is assessed by fluorescence microscopy, Alamar blue and other methods, and hemoglobin content in the lipoaspirate is determined.
In vitro model of normal intestinal tissue
A 2D/3D in vitro intestinal model formed from colonic stem cells for testing various active substances. It can assess morphological changes, proliferation, apoptosis markers, changes in gene and protein expression, epithelial barrier function.
