Lithuanian University of Health Sciences Research Management System (CRIS)





DSpace-CRIS 8

Recent Additions
  • preprint
    Cesanelli, L.
    ;
    Minderis, P.
    ;
    ;
    Ratkevičius, A.
    ;
    Degens, H.
    ;
    Satkunskienė, D.
    Cell and Tissue Research, 2025-03-31, vol. 00, no. 00, p. 1-16

    Obesity has been associated with lower muscle strength-to-body mass ratio. Here, we evaluated the effects of diet-induced obesity on the mechano-structural properties of isolated muscles and tendons. Thirty 10-week-old male C57BL/6 J mice were randomly assigned to either an obesogenic high-fat diet group (OB) for 24 weeks or a control group (CN) maintained on a standard chow diet. Soleus muscle (SOL) and Achilles tendon (AT) specimens were isolated and subjected either to failure testing, 300 cycles of passive stretch-destretch, or isometric twitch contractions. Morpho-structural and protein expression analyses were conducted to assess collagen and adipose tissue accumulation, concentrations of cross-linking factors, and any alterations in the POSTN-TGFβ1-Akt signaling pathway. OB SOL and AT tissues were more fragile than those from CN (p < 0.05). A piecewise linear regression model revealed a tendency for OB tissues to exhibit steeper mechanical property changes within the first 20 cycles compared to CN, followed by a similar plateau phase in both groups. OB SOL-AT complexes showed a slower twitch-contraction-relaxation pattern than CN (p < 0.05). OB tendons and muscles were larger than those of the CN, with muscles featuring bigger fibers, and higher collagen area fraction (p < 0.05). Elevated TGFβ1 and POSTN concentrations were observed in OB tissues (p < 0.05), alongside increased P-Akt and P-4EBP1 expression (p < 0.05). These findings highlight the detrimental effects of obesity on the structural integrity of muscle and tendon tissues and suggest a significant role of POSTN-TGFβ1-Akt signaling in obesity-associated musculotendinous remodeling.

  • research article
    Anaya, Brayan J.
    ;
    ;
    Ureña-Vacas, Isabel
    ;
    Sanz-Perez, Amadeo
    ;
    ; ;
    García-Rodríguez, Juan José
    ;
    Serrano, Dolores R.
    ;
    González-Burgos, Elena
    Gels, 2025-03-26, vol. 11, no. 4, p. 1-21

    This study investigates the phytochemical profile, antioxidant and anti-inflammatory properties, and 3D-printing application of Origanum vulgare L. ssp. hirtum extract. The extract revealed a diverse range of phenolic compounds, with rosmarinic acid as the predominant compound (47.76%). The extract showed moderate to high lipoxygenase inhibition (IC50 = 32.0 µg/mL), suggesting its potential as an anti-inflammatory agent. It also exhibited strong antioxidant activity, with hydrogen peroxide scavenging (SC50 = 99.2 µg/mL) and hydroxyl radical scavenging (IC50 = 64.12 µg/mL) capabilities. In cellular studies, high concentrations (50 µg/mL and 100 µg/mL) significantly decreased intracellular ROS production in Caco-2 cells (reductions exceeding 53% and 64%, respectively). Moreover, the extract suppressed NO production in LPS-stimulated J774A.1 macrophages in a concentration-dependent manner. The study also explores the incorporation of the extract into 3D-printed gummies. The gels exhibited a shear-thinning behavior, which was essential for successful extrusion-based 3D printing. The incorporation of Origanum extract significantly influenced the mechanical strength and compaction properties of the 3D-printed gummies before breaking (1.6-fold increase) allowing for a better mouth feeling. PXRD and FTIR analyses confirmed the amorphous nature of the 3D-printed gummies and the interaction between active ingredients and excipients utilized for printing. These findings demonstrated the potential for semisolid extrusion 3D printing at room temperature to transform a culinary herb (Origanum vulgare spp. hirtum) into a healthcare product with antioxidant and anti-inflammatory properties.

  • research article
    Tumosienė, Ingrida
    ;
    Stasevych, Maryna
    ;
    Zvarych, Viktor
    ;
    Jonuškienė, Ilona
    ;
    Kantminienė, Kristina
    ;
    International Journal of Molecular Sciences, 2025-03-29, vol. 26, no. 7, p. 1-21

    A series of novel hydrazones bearing diphenylamine and 5-oxopyrrolidine moieties, along with benzene and naphthalene rings substituted with hydroxy, alkoxy, or carboxylic groups, were synthesized. Their anticancer activity was evaluated in vitro using both 2D (MTT and ‘wound healing’ assays) and 3D (cell spheroid) models against human melanoma IGR39 cells, the triple-negative breast cancer cell line MDA-MB-231, and pancreatic carcinoma Panc-1 cell line. Compounds 8 (2-hydroxybenzylidene derivative) and 12 (2-hydroxynaphthalenylmethylene derivative) demonstrated the highest cytotoxicity in both 2D and 3D assays, while compounds 4 (2,5-dimethoxybenzylidene derivative) and 6 (2,4,6-trimethoxybenzylidene derivative) were most effective at inhibiting cell migration. Notably, all compounds exhibited lower activity against the Panc-1 cancer cell line in a cell monolayer, but the effects on spheroid cell viability in 3D models were comparable across all tested cancer cell lines. Molecular docking studies of the most active hydrazones suggested that these compounds may act as multikinase inhibitors. In particular, 2-hydroxynaphthalenylmethylene derivative 12 showed high binding affinity values (−11.174 and −11.471 kcal/mol) to the active sites of two key protein kinases—a non-receptor TK (SCR) and STPK (BRAF)—simultaneously.

  • research article
    Kasprowicz, Magdalena
    ;
    Mataczyński, Cyprian
    ;
    Uryga, Agnieszka
    ;
    Pelah, Adam I
    ;
    Schmidt, Eric
    ;
    Czosnyka, Marek
    ;
    Kazimierska, Agnieszka
    ;
    CENTER-TBI High-Resolution ICU (HR ICU) Sub-Study Participants and Investigators
    ;
    Ragauskas, Arminas
    ;
    Rocka, Saulius
    ;
    ;
    Critical Care (London, England), 2025-02-17, vol. 29, no. 1, p. 1-12

    Morphological analysis of intracranial pressure (ICP) pulse waveforms provides indirect information on cerebrospinal compliance, which might be reduced by space-occupying lesions but also by intracranial hypertension and aging. This study investigates the impact of age and mean ICP on the shape and amplitude of ICP pulse waveform in traumatic brain injury (TBI). Additionally, it explores the association between morphological parameters and mortality after TBI.

  • research article
    Ritzer, Lukas
    ;
    Harris, Alon
    ;
    Arciero, Julia
    ;
    Siesky, Brent
    ;
    Fry, Brendan
    ;
    ;
    Oddone, Francesco
    ;
    Carnevale, Carmela
    ;
    Eckert, George
    ;
    Verticchio Vercellin, Alice
    Journal of Glaucoma, 2025-04-01, vol. 34, no. 4, p. 267-274

    The relationship between structural and hemodynamic parameters in patients with primary open angle glaucoma is strongest in the temporal region of the optic nerve.

Most viewed
  • Universitetas / University
      9234
  • Researcher
    Stankutė, Deimantė
    Istorija / History
    Some of the metrics are blocked by your 
      6920
  • Researcher
    Kavaliauskaitė, Agnė
    Istorija / History
    Some of the metrics are blocked by your 
      5391  2
  • Some of the metrics are blocked by your 
      5239
  • journal article
    Lietuvos bendrosios praktikos gydytojas. Kaunas : Vitae Litera, 2008, t. 12, Nr. 10., p. 679-681.

    Lėtinis atrofinis gastritas – tai ilgalaikio lėtinio skrandžio uždegimo sąlygota skrandžio gleivinės atrofija. Atrofinio gastrito fone vystosi metaplazija žarninio tipo epiteliu, vėliau epitelio displazija ir skrandžio vėžys. Atrofija ir žarninė metaplazija apibūdinamos kaip ikivėžinės būklės. Skrandžio gleivinės atrofija ir žarninė metaplazija yra dažnesnės šalyse, kuriose yra didelis sergamumas skrandžio vėžiu. Atrofinis gastritas laikomas ikivėžine liga, tačiau nėra visiškai aišku, ar galimas atrofijos proceso sustabdymas, ar tai leidžia apsaugoti nuo skrandžio vėžio.

      4640
Most downloaded