Lithuanian University of Health Sciences Research Management System (CRIS)





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  • preprint; ;
    Simanavičius, Martynas
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    Tamošiūnas, Paulius Lukas
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    Vaccine, 2024-11-30, vol. 00, no. 00, p. 1-6

    Hepatitis E virus genotype 3 (HEV-3) is a zoonotic pathogen capable of infecting human, porcine, and other animal hosts. Despite a broad host range and abundance of species that act as reservoirs for human infections, no commercially available animal vaccines against HEV-3 are currently available. In the present study, we tested the capacity of recombinant aa 112-608 wild boar-derived HEV-3 capsid protein (rORF2p) to induce an immune response in immunized pigs. Four 6 week old pigs were administered 1 ml of 200 μg/ml rORF2p, followed by booster administration after 14 days. Blood samples were collected until 28 days after initial immunization. Dominant cell phenotypes and anti-HEV IgG concentrations were determined. A significant anti-HEV IgG, monocyte/macrophage, B cell and T cell response has been detected in immunized pigs. In turn, our findings suggest the capacity of rORF2p to elicit an immune response in pigs, suggesting the potential for its use as a vaccine candidate.

  • research article
    Riachi, Emile
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    The International journal of oral & maxillofacial implants, 2024-10-16, vol. 39, no. 5, p. 157-173

    Purpose: To assess the impact of implant placement at different time intervals on the esthetic and clinical outcomes in the esthetic zone. Materials and Methods: A literature screening was conducted in PubMed (MEDLINE), ScienceDirect, and Cochrane databases. Relevant articles were included according to selection criteria and Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines. Data was collected from studies published from 2017 to 2022 in English. Results: Nine articles were included, in which a total of 495 implants were placed; 250 of the implants were immediate, 109 were early, and 136 were delayed. Immediate implant placement (IIP) showed no statistically significant difference in Pink Esthetic Score (PES) compared with delayed implant placement (DIP). IIP showed significantly higher PES in comparison with early implant placement (EIP) (mean difference [MD] = –0.76; 95% CI = –1.50 to –0.02; P = .04). The probing depth (PD) was considerably greater for immediate implants than for delayed implants (MD = –0.62; 95% CI = –1.05 to –0.18; P = .005), and the Plaque Index (PI) was statistically greater for early implants compared with immediate implants (MD = 0.15; 95% CI = 0.11 to 0.19; P < .00001). All other soft tissue outcomes showed equal results. The marginal bone loss (MBL) was statistically higher in early implants compared with immediate implants (MD = 0.09; 95% CI = 0.02 to 0.16; P = .02). Conclusions: IIP had significantly superior PES, MBL, and PI results when compared with EIP. The PD was significantly higher for immediate implants compared with delayed implants. All other outcomes showed no significant difference between the three implant groups. It is important to highlight the limitations of this review such as the small number of studies included and the few reports on esthetic indices.

  • research article;
    Rajackaitė, Ema
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    Robbins, Ninette
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    Ali, Mohamed
    Annals of Blood, 2024-09-30, vol. 9, p. 1-10

    Background: There are significant benefits for patients receiving plasma or plasma derived medicinal products, but for donors undergoing plasmapheresis, benefits are poorly researched and defined. A study by Rosa-Bray et al. [2013] describes findings of reduced levels of low-density lipoprotein (LDL) and increased yield of high-density lipoprotein after plasmapheresis in donors’ samples. Our article aims to address some of the feasible investigative options, based on Rosa-Bray’ findings, by proposing new theoretical computing “Predictive Model of variable laboratory findings calculation and monitoring during plasmapheresis procedures”, which highlights cholesterol metabolism changes. Methods: Literature review was performed using National Center for Biotechnology Information (NCBI) Literature Resources based on thematic analysis with keywords: “lipoproteins in plasma”, “cholesterol chemistry”, “plasma donors and lipoproteins”, “plasmapheresis and lipids”. Filters for full text, original study, review and metanalysis, not older than 10 years were selected. The literature search was performed during 5 March to 10 June 2024. Seventy-six available sources were found and 58 were left for final revision. Custom programming with web technologies was used—HTML, CSS, Javascript. Following libraries were used to create the program—amCharts, Google Charts, Bootstrap. All programming work was done with Visual Studio Code software. Results: The created and proposed theoretical model can be accessed using active link—http://lpmc.labdata.lt/. It was developed to show the beneficial possibilities if installed in the software during plasmapheresis and after. Further development to obtain the model into routine practice would work on actual donor data with additional features for microelements. Conclusions: The created theoretical model will work for laboratory monitoring serial results during the plasmapheresis procedures with demonstration of possible changes in plasma highlights the potential benefits of plasmapheresis. The Predictive Model emphasizes possible insights for cholesterol metabolism with further proposals on proteins, microelements and enzymes monitoring.

  • conference paper; ; ; ;
    The 13th International Conference of Young Scientists "Young Scientists for Advance of Agriculture" AGRISCI2024 : Abstracts, 2024-11-26, p. 8-8

    In cows, lactose is the primary solid component of milk and is routinely measured in lactating cows worldwide as part of standard evaluation systems. Over recent decades, likely due to the growing availability of milk data from innovative technologies, lactose has been increasingly incorporated into scientific studies, alongside traditional traits like milk yield, fat, and protein concentrations. The goal of this study was to determine the connection between in-line lactose concentration and rumination quality. Research was conducted on a Lithuanian farm in 2023. A total of 502 cows were selected for the study. According to the literature (1) they were divided into two groups: A (lactose concentration < 4.7%) and B (lactose concentration ≥ 4.7%). The average milk yield was 12,500 kg per lactation. Milk lactose concentration was recorded continuously during each milking by the “BROLIS HERDLINE (Vilnius, Lithuania)” in-line milk analyzer, which was integrated into a milking robot. Rumination quality was monitored using the RumiWatch sensor (Itin + Hoch GmbH, Liestal, Switzerland). Jaw movements during rumination showed a significantly higher number of rumination chews (p < 0.01), with a 13.84% increase in Group B (ML ≥ 4.70%) compared to Group A (ML < 4.70%). The average number of rumination chews in Group B was 1280.80 chews per hour (±52.28), while in Group A, it was 1103.58 chews per hour (±23.37). Additionally, the results showed a significant increase (p < 0.001) of 16.70% in the number of cuds in Group B (ML ≥ 4.70%) compared to Group A (ML < 4.70%). The average number of cuds in Group B was 22.69 per hour (±0.92), while in Group A, it was 18.90 per hour (±0.38). Our study has shown that lactose concentration has a strong relation with rumination quality and may provide insight into cattle health status.

  • Publicationagris
    conference paper
    The 13th International Conference of Young Scientists "Young Scientists for Advance of Agriculture" AGRISCI2024 : Abstracts, 2024-11-26
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