A Construct for Articular Cartilage Regeneration and a Method of Preparation (EP 4119169 B1)

Abstract

The base of the regeneration construct scaffold is a biodegradable polymer. The biodegradable polymer is blended with natural additives, such as cellulose and hydroxyapatite which increase tissue affinity. The regeneration construct is composed of two layers, where layer I comprises blend of biodegradable polymer and cellulose and is prepared for cartilage tissue formation; and layer II comprises blend of biodegradable polymer, cellulose, and hydroxyapatite and is prepared for subchondral bone tissue formation. A method of preparation of construct comprises preparation of polymer solutions for layers I and II, applying of solution cryo-electrospinning, conversion of cellulose acetate to cellulose, surface modification of the regeneration construct layer I scaffold by treating with ozone, embedding of biologically active molecules in the regeneration construct scaffold layer I; sterilization of the regeneration construct layer I and II scaffold and combining of the regeneration construct layer I and II scaffolds in order to form final regeneration construct.