Recent Additions
  • Background and Objectives: Vaccination is one means of SARS-CoV-2 prevention and control. However, despite the effectiveness of vaccination, adverse reactions continue to require vigilance and monitoring. The researchers emphasize the possibility that some of the reported side effects may be psychological in origin. Based on this hypothesis, the main goal of this study was to evaluate the emotional dispositions of healthcare workers who experienced emotions before vaccination and adverse reactions after vaccination. Materials and Methods: This study was conducted between February and May 2021 in the Kaunas Clinics of the University of Health Sciences. A total of 2117 employees of the clinic departments who were vaccinated with two doses of the Pfizer–BioNTech vaccine participated in this study. Statistical analysis was performed on the data using IBM SPSS Statistics®. Results: Most participants (74.5%) experienced systemic (including local) adverse events; 16.5% experienced only local adverse events, and 9.1% experienced no adverse events. The frequency of systemic (including local) adverse events reduced with increasing age (p<0.05). The main emotions that participants experienced before vaccination were anxiety (37.88%) and happiness (39.02%). Systemic (including local) adverse events occurred 1.26 times more frequently in women than men (77.44% vs. 61.6%, p<0.05), while local adverse events occurred 1.4 times more often in male participants than in female participants (21.39% vs. 15.27%, p<0.05). Among the respondents who did not experience adverse events, the most common emotion felt was happiness (25.5%), and most of the participants who experienced systemic (including local) adverse events felt anxiety (42.6%). Conclusions: The information about vaccination and potential adverse events should be targeted at younger persons. It is recommended that women, more than men, should receive professional counseling from psychologists or psychotherapists. The public dissemination of positive messages about the benefits and safety of vaccines prior to a vaccination campaign may alleviate the tension or anxiety felt regarding potential adverse events. Healthcare specialists—both those who work directly with vaccines and those who do not—should maintain a positive psychological attitude towards vaccination, as this can increase patient satisfaction with the benefits of vaccines.
  • research article
    Sepp, Janne
    ;
    Koshovyi, Oleh
    ;
    ; ;
    Botsula, Iryna
    ;
    Kireyev, Igor
    ;
    Tsemenko, Karina
    ;
    Kukhtenko, Oleksandr
    ;
    Kogermann, Karin
    ;
    Heinämäki, Jyrki
    ;
    Raal, Ain
    Plants
    Galenic preparations of German chamomile are used to treat mild skin diseases, inflammation, and spasms, and they have also been reported to have anxiolytic and sedative effects. The medicinal use of chamomile is well known in ethnomedicine. After obtaining its galenic preparations, there is lots of waste left, so it is expedient to develop waste-free technologies. The aims of this study were to gain knowledge of the ethnomedical status of chamomile in the past and present, develop methods for preparing essential oils and dry extracts from German chamomile flowers using complex processing, reveal the phytochemical composition of such extracts, and verify the analgesic and soporific activity of the extracts. Two methods for the complex processing of German chamomile flowers were developed, which allowed us to obtain the essential oil and dry extracts of the tincture and aqueous extracts as byproducts. A total of 22 phenolic compounds (7 hydroxycinnamic acids, 13 flavonoids, and 2 phenolic acids) were found in the dry extracts by using UPLC-MS/MS. In total, nine main terpenoids were identified in the chamomile oil, which is of the bisabolol chemotype. During the production of chamomile tincture, a raw material–extractant ratio of 1:14–1:16 and triple extraction are recommended for its highest yield. In in vivo studies with mice and rats, the extracts showed analgesic activity and improvements in sleep. The highest sedative and analgesic effects in rodents were found with the dry extract prepared by using a 70% aqueous ethanol solution for extraction at a dose of 50 mg/kg. The developed methods for the complex processing of German chamomile flowers are advisable for implementation into the pharmaceutical industry to reduce the volume of waste during the production of its essential oil and tincture, and to obtain new products.
  • research article
    Rose, Angela M C
    ;
    Pozo, Francisco
    ;
    Martínez-Baz, Iván
    ;
    Mazagatos, Clara
    ;
    Bossuyt, Nathalie
    ;
    Cauchi, John Paul
    ;
    Petrović, Goranka
    ;
    Loghin, Isabela I
    ;
    ;
    Buda, Silke
    ;
    Machado, Ausenda
    ;
    Duffy, Róisín
    ;
    Oroszi, Beatrix
    ;
    Howard, Jennifer
    ;
    Echeverria, Aitziber
    ;
    Andreu, Cristina
    ;
    Barbezange, Cyril
    ;
    Džiugytė, Aušra
    ;
    Nonković, Diana
    ;
    Popescu, Corneliu-Petru
    ;
    Majauskaitė, Fausta
    ;
    Tolksdorf, Kristin
    ;
    Gomez, Verónica
    ;
    Domegan, Lisa
    ;
    Horváth, Judit Krisztina
    ;
    Castilla, Jesús
    ;
    García, Miriam
    ;
    Demuyser, Thomas
    ;
    Borg, Maria-Louise
    ;
    Tabain, Irena
    ;
    Lazar, Mihaela
    ;
    Kubiliutė, Ieva
    ;
    Dürrwald, Ralf
    ;
    Guiomar, Raquel
    ;
    O'Donnell, Joan
    ;
    Kristóf, Katalin
    ;
    Nicolay, Nathalie
    ;
    Bacci, Sabrina
    ;
    Kissling, Esther
    ;
    VEBIS SARI VE network team
    ;
    ; ;
    Jancorienė, Ligita
    ;
    Zablockienė, Birutė
    Influenza and Other Respiratory Viruses
    We conducted a multicentre hospital-based test-negative case-control study to measure vaccine effectiveness (VE) against PCR-confirmed influenza in adult patients with severe acute respiratory infection (SARI) during the 2022/2023 influenza season in Europe. Among 5547 SARI patients ≥18 years, 2963 (53%) were vaccinated against influenza. Overall VE against influenza A(H1N1)pdm09 was 11% (95% CI: -23-36); 20% (95% CI: -4-39) against A(H3N2) and 56% (95% CI: 22-75) against B. During the 2022/2023 season, while VE against hospitalisation with influenza B was >55%, it was ≤20% for influenza A subtypes. While influenza vaccination should be a priority for future seasons, improved vaccines against influenza are needed.
      2WOS© IF 4.4WOS© AIF 6Scopus© SNIP 1.043
  • research article
    Kairytė, Karolina
    ;
    Vaickelionienė, Rita
    ;
    Grybaitė, Birutė
    ;
    Anusevičius, Kazimieras
    ;
    Mickevičius, Vytautas
    ;
    International Journal of Molecular Sciences
    A series of hydrazones, azoles, and azines bearing a 4-dimethylaminophenyl-5-oxopyrrolidine scaffold was synthesized. Their cytotoxic effect against human pancreatic carcinoma Panc-1 and triple-negative breast cancer MDA-MB-231 cell lines was established by MTT assay. Pyrrolidinone derivatives 3c and 3d, with incorporated 5-chloro and 5-methylbenzimidazole fragments; hydrazone 5k bearing a 5-nitrothien-2-yl substitution; and hydrazone 5l with a naphth-1-yl fragment in the structure significantly decreased the viability of both cancer cell lines. Compounds 3c and 5k showed the highest selectivity, especially against the MDA-MB-231 cancer cell line. The EC50 values of the most active compound 5k against the MDA-MB231 cell line was 7.3 ± 0.4 μM, which were slightly higher against the Panc-1 cell line (10.2 ± 2.6 μM). Four selected pyrrolidone derivatives showed relatively high activity in a clonogenic assay. Compound 5k was the most active in both cell cultures, and it completely disturbed MDA-MB-231 cell colony growth at 1 and 2 μM and showed a strong effect on Panc-1 cell colony formation, especially at 2 μM. The compounds did not show an inhibitory effect on cell line migration by the ‘wound-healing’ assay. Compound 3d most efficiently inhibited the growth of Panc-1 spheroids and reduced cell viability in MDA-MB-231 spheroids. Considering these different activities in biological assays, the selected pyrrolidinone derivatives could be further tested to better understand the structure–activity relationship and their mechanism of action.
      1WOS© IF 5.6WOS© AIF 6.4Scopus© SNIP 1.263
  • In this study, we examined 130 patients with pituitary adenomas (PAs) and 320 healthy subjects, using DNA samples from peripheral blood leukocytes purified through the DNA salting-out method. Real-time polymerase chain reaction (RT-PCR) was used to assess single nucleotide polymorphisms (SNPs) and relative leukocyte telomere lengths (RLTLs), while enzyme-linked immunosorbent assay (ELISA) was used to determine the levels of TERF1, TERF2, TNKS2, CTC1, and ZNF676 in blood serum. Our findings reveal several significant associations. Genetic associations with pituitary adenoma occurrence: the TERF1 rs1545827 CT + TT genotypes were linked to 2.9-fold decreased odds of PA occurrence. Conversely, the TNKS2 rs10509637 GG genotype showed 6.5-fold increased odds of PA occurrence. Gender-specific genetic associations with PA occurrence: in females, the TERF1 rs1545827 CC + TT genotypes indicated 3.1-fold decreased odds of PA occurrence, while the TNKS2 rs10509637 AA genotype was associated with 4.6-fold increased odds. In males, the presence of the TERF1 rs1545827 T allele was associated with 2.2-fold decreased odds of PA occurrence, while the TNKS2 rs10509637 AA genotype was linked to a substantial 10.6-fold increase in odds. Associations with pituitary adenoma recurrence: the TNKS2 rs10509637 AA genotype was associated with 4.2-fold increased odds of PA recurrence. On the other hand, the TERF1 rs1545827 CT + TT genotypes were linked to 3.5-fold decreased odds of PA without recurrence, while the TNKS2 rs10509637 AA genotype was associated with 6.4-fold increased odds of PA without recurrence. Serum TERF2 and TERF1 levels: patients with PA exhibited elevated serum TERF2 levels compared to the reference group. Conversely, patients with PA had decreased TERF1 serum levels compared to the reference group. Relative leukocyte telomere length (RLTL): a significant difference in RLTL between the PA group and the reference group was observed, with PA patients having longer telomeres. Genetic associations with telomere shortening: the TERF1 rs1545827 T allele was associated with 1.4-fold decreased odds of telomere shortening. In contrast, the CTC1 rs3027234 TT genotype was linked to 4.8-fold increased odds of telomere shortening. These findings suggest a complex interplay between genetic factors, telomere length, and pituitary adenoma occurrence and recurrence, with potential gender-specific effects. Furthermore, variations in TERF1 and TNKS2 genes may play crucial roles in telomere length regulation and disease susceptibility.
      3WOS© IF 5.2WOS© AIF 5.7Scopus© SNIP 1.086
Most viewed
  • Administracinis / Administrative
      4320
  • conference paper
    7th Japan-Lithuania Joint Life Sciences Symposium: New approach to Life Sciences“ : 18 December, 2020 / Lithuanian University of Health Sciences. Kaunas : Lithuanian University of Health Sciences, 2020., p. 1-1.
    Voice disorders (hoarseness) are a common condition manifesting in almost 10% of the population. The causes of hoarseness can be related to common respiratory diseases and/or to vocal fatigue caused by vocal overload, however it can also be related to functional, behavioral, neurologic factors and both benign and malignant laryngeal tumors. Hoarseness can also be an early symptom of laryngeal cancer. The VoiceScreen app requires the user to perform two simple tasks: 1). Sustain a vowel [a:] sound for 4 seconds, 2) Read the provided sentence. The users’ voice is recorded and then analyzed extracting six acoustic voice function parameters which are then weighted and merged together to provide the user with the Acoustic Voice Quality Index (AVQI) of his/her voice. The value of AVQI as a clinical marker of overall voice quality has already been proven across multiple studies revealing adequate diagnostic accuracy. The AVQI values do not depend on gender and age. The VoiceScreen app calculates and displays the value of AVQI and also provides the user with recommendations based on the test results. The provided recommendations should be taken as guidance on what to do next to help the user’s voice. [...].
      3644
  •   2202
  • Researcher
    Subočius, Andrėjus
    Istorija / History
      2182
  •   1621
Most downloaded