The Effect of modified myoblasts to function and cardiac muscle regeneration in rabbit’s acute myocardial infarction model
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2012-08-16 |
Study Objective The aim of study was to evaluate skeletal myoblast (SMs) and SMs expressing connexin 43 (Cx43-EGFP) therapy impact to the functional recovery of the acutely injured heart muscle in rabbit model. Methods And Material We used New Zealand white rabbits aged 6 months, weighing 3.5 kg. SMs were isolated from the femoral muscle biopsy and cultured in the IMDM with 10% FBS. Myocardial infarction (MI) was induced by ligation of the LAD coronary artery. Cell implantation was performed by injection of 0.5 ml cell suspension with 6 M SMs into MI zone through a 30-gauge needle. Animals were divided into 4 groups: 1) control (n=5) 2) sham injected (n=5) 3) SM injected 4) injected with SMs Cx43-EGFP (n=5). The heart ejection fraction (EF) was evaluated before and 1 and 3 months after MI by echocardiogram. Results After MI, EF decreased in all groups. During 3 months after MI, EF continued to decrease in the control, sham and SM group with LV aneurism forming. In the SM Cx43-EGFP group reduction of EF was smallest with partial recovery after 3 months with restoration of LV segments in MI zone. Conclusions The beneficial effect of cellular therapy on EF was observed in the Cx43-EGFP expressing myoblasts group.