The Association between miR-328 expression of whole blood and myopia

Abstract

Purpose The aim of this study is to find associations between miR-328 expression of whole blood and myopia. Methods 112 individuals (42 individuals with moderate or high myopia and 70 healthy individuals) were evaluated. RNA was extracted from peripheral blood samples and expression of miR-328 was assessed by using the Applied Biosystems 7900HT Real-Time Polymerase Chain Reaction System. Refractive error was measured with Sol. Cyclopentolate 1% using an autorefractor (Accuref-K9001, Shin-Nippon, Japan) and calculated by the mean spherical equivalent for each of the two eyes of every individual. Results The ΔΔCT values of miR-328 were compared between the myopic and the control groups. The results showed that ΔΔCT values differ statistically significantly between these two groups (p<0.05). In myopia group, ΔΔCT of miR-328 values are lower than in control group. We found that the area under the ROC curve (AUC) of miR-328 is 0.631, 95% CI 0.522-0.739. Sensitivity varied from 26.2 % to 73.8% and specificity from 34.3 % to 65.7%. Conclusions miR-328 expression level of whole blood statistically significantly differ between myopia and control groups.