Contamination of poultry environment with campylobacter spp. and significance for colonisation of broilers
| Date Issued |
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2016-10-06 |
ISBN 978-9955-15-448-8.
Konferencija skirta Lietuvos sveikatos mokslų universiteto Veterinarijos akademijos įkūrimo 80–čiui ir Lietuvos sveikatos mokslų universiteto Veterinarijos akademijos Gyvulininkystės technologijos fakulteto įkūrimo 70-čiui.
Various animal species, wild birds, rodents and pets are the main source of Campylobacter contamination in broiler farms. However, little is known about the ability of these bacteria to survive outside the host and their role in the colonisation of broilers. Therefore, in this study we aimed to identify the sources of Campylobacter in poultry farm environment and their significance for broiler colonisation. For this purpose, Campylobacter spp. were isolated from broilers and their environment (Litter, water of puddle, concentric zones, tap water) by both direct inoculation on mCCDA selective medium and selective enrichment into Exeter broth. Campylobacter isolates were identified to the species level by multiplex polymerase chain reaction. The genetic diversity of Campylobacter jejuni strains isolated from broilers and the environment was examined by PCR-based restriction fragment lenght polymorphism analysis of PCR amplified portion of the flagellin-A (flaA) gene. [...].