Evaluation of pharmaceutical factors influence to the extraction of phenolic compounds
| Author(s) | ||
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National University of Pharmacy, Kharkiv, Ukraine | ||
| Date Issued |
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2022-10-21 |
Plenary Session. Abstracts. Scientists session
ISBN 978-9955-15-769-4
Introduction: Extraction is an essential step in the study of natural biologically active substances of herbal origin. Classical and innovative methods are used for extraction, factors affecting the efficiency of the processes are studied widely. Scientific data confirm the relevance of choosing the extraction method and the most suitable conditions. The aim of this study is to evaluate the factors that can influence the herbal extraction quality and determine the amount of phenolic compounds and antioxidant activity of tested extracts. Materials and methods: Analysed raw materials were herb of purple coneflower and horsetail, leaves of lemon balm and broadleaf plantain. Ultrasound extraction (US) was performed. Pharmaceutical factors: different concentrations of ethanol (50 – 90%); different material and solvent ratio (1:10, 1:20, 1:30); different duration of extraction (10 min, 20 min, 30 min, 40 min, 50 min.) were applied in the experimental studies. The total amount of phenolics (TPC) was determined spectrophotometrically by Folin-Ciocalteu reagent, concentration expressed in gallic acid equivalents (GAE mg/mL). The antioxidant activity was evaluated using spectrophotometric DPPH radical scavenging method, results expressed as µg/mL Trolox equivalents (TE). Results: The highest content of phenolic compounds was 399.5 GAE mg/mL and 211.42 µg/mL TE antioxidant activity in 60% ethanolic broadleaf plantain extract after 30 min extraction in 1:30 ratio. The maximum amount of TPC was extracted using a solvent of ethanol 40%, ratio of 1:30, after 30 min. extraction time using herb of purple coneflower. 50% ethanolic extract of lemon balm contained highest amount of TPC after 15 min extraction in ratio 1:10. The highest content of TPC 23.93 GAE mg/mL and highest antioxidant activity was 25.2 µg/mL TE was determined in 70% ethanolic horsetail extract at ratio 1:30 after 20 min ultrasound extraction. Conclusions: The obtained results confirm the importance of suitable solvent, herbal material and solvent ratio and duration of extraction process. The search, technology and quality of new herbal preparations is an important part of pharmaceutical science.