Fresh wound treatment of the dog using platelet-rich fibrin

Abstract

The platelet-rich fibrin membrane contains a fibrin matrix with leukocytes, erythrocytes, platelets, B and T lymphocytes, monocytes, stem cells and growth factors which provide better and faster tissue regeneration. Because of those features, PRF membrane is very useful in a contaminated and fresh wound treatment [1]. The clinical history was the following: a 12 month old female Curly-coated retriever dog, weight 24 kg BMI=3, was admitted to the the clinic for a fresh wound treatment, and then during a physical examination, a wound (10 mm x 13 mm) was noticed on a metacarpal pad (torus metacarpalis) of the front leg without any purulent discharge. The method applied: the wound was cleaned with chlorhexidine 0.12% solution. The blood sample (10 ml) was collected from v. saphena into PRF sterile tubes without any anticoagulant. The WB sample was centrifuged on 2500 rpm, for 12 min. After separation and compression, the PRF membrane was put in a wound area and bandaged. Control re-checks and re-bandage were performed 5, 14 and 25 days after the procedure. For evaluation, the wound healing score system [2] and wound measurement were used. During the first control check-up (after 5 days), the wound was without purulent discharge, a visible reepithelisation margin, and its size was 8 mm x 8 mm (by 50% smaller compared to the first day). 14 days after the procedure, on the second control check-up, the wound had no purulent discharge, a wide margin of re-epithelisation, and its size was 3 mm x 3 mm (by 93% smaller compared to the first day). 25 days after the procedure, the wound was totally closed up, with no purulent discharges, and a small connective tissue scar was visible on the metacarpal pad. To conclude, PRF may be a useful tool for wound treatment which helps to extend time between bandaging and to reduce the use of antibiotics, but there is a need for more thorough research of this concept for treatment of ...[...].