Post-extraction site wound healing using Platelet-Rich-Fibrin in dogs

Abstract

Introduction. Periodontal disease is one of the most common condition inb dogs and dental extraction is usually required [1]. Platelet-Rich Fibrin (PRF) membranes are widely and successfully used in the oral surgery of humans. Platelet-rich-fibrin membrane contains a fibrin matrix with leukocytes, erythrocytes, platels, B and T lymophocytes, monocytes, stem cells and growth factors which provides better and faster tissue regenerating [2]. Faster wound healing helps to reduce the amount of nonsteroidal anti-inflammatory drugs and makes general comfort for the dog. Methods. Clinical condition and periodontal disease stage were evaluated in the group of 11 dogs that had no any other chronic disease. The dogs were split in two groups (n1=6; N2=5): the procedure of tooth extraction and use of platelet-rich fibrin (n1=6); and procedure of standard tooth extraction (n2=5). Procedure of closed dental extraction was made under general anaesthesia. Oral cavity was cleaned with chlorhexidine 0,12% solution. Whole blood sample (5 ml) was colected from saphenous vein into the sterile tube without anticoagulant. WB sample was centrifuged on 2400 rpm, 12 min., 500 g. After separation and compression, PRF membrane was put and fixated in the alveolar wound of the first group dogs (n1). [...].