Blood clot and platelet-rich fibrin resistance against microorganism induced fibrinolysis

Abstract

Introduction Alveolitis still remains one of the most common late post-extractional complications, which is diagnosed 1-4% of all cases and reaches significant 37,5% while removing third mandibular molars. Various microorganisms, including Bacillus cereus, Streptococcus pneumoniae, Staphylococcus aureus, Candida albicans, release endotoxins and induce the lysis of the blood clot which results as an inflammation of alveolis. Platelet rich fibrin (PRF) consists of great number of growth factors, cytokines and platelets, which reduce the inflammatory process. Aim To evaluate PRF and blood clot resistance against microorganism induced fibrinolysis. Objectives 1. Quantitative and qualitative evaluation of Bacillus cereus, Streptococcus pneumoniae, Staphylococcus aureus, Candida albicans fibrinolytic activity in blood clot 2. Quantitative and qualitative evaluation of Bacillus cereus, Streptococcus pneumoniae, Staphylococcus aureus, Candida albicans fibrinolytic activity in PRF. 3. Compare concentration of D-dimers in both mediums after 1 and 3 hours of incubation. Methods To perform this study a permission from LSMU Bioethics center was received. Blood samples were taken from 40 young and healthy volunteers. For each of them three types of test-tubes were used: 1) A- containing trisodium citrate, 4,5 ml (blue). 2) B- without additional reagents, 5 ml (red). 3) C – PRF vacutainer, 10 ml (brown). Samples were divided in 4 groups, 10 volunteers each. Every group underwent the experiment with one of the following microorganisms: Bacillus cereus, Streptococcus pneumoniae, Staphylococcus aureus, Candida albicans. Tube A immediatly after blood drawing procedure was centrifuged for 9 minutes, 2500 RCF (Hettich Rotina 35, Germany). This is how blood plasma is made. After 3 hours of incubation in room temperature, concentration of D- dimers was measured (STAGO Diagnostics, STA Compact, France). Tube B was kept in room temp.[...].