Effect of iron sulphate and iron glycinate on short chain fatty acid (SCFA) concentration in broiler chickens’ caecum
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2016-09-27 |
ISBN-978-83-933604-7-5.
Introduction: Iron is vital for almost all living organisms as it participates in a wide range of metabolic processes. However, iron concentration in body tissues must be tightly regulated since the excessive iron accumulation may lead to microbial infections or can cause tissue damage. Intestinal microbiota and their metabolic end product, especially short chain fatty acids (SCFA), play an important role in maintaining homeostasis in the gastrointestinal tract (GIT). The previous studies have demonstrated that SCFA affected intestinal motility by nerve and muscle stimulation. Moreover, the intestinal mucus secretion and cell proliferation were affected by luminal SCFA. In addition, besides their beneficial effects on GIT structure and function, SCFA have been shown to control the whole luminal microbial ecology. Aim: So, the aim of the trial was to investigate the effect of iron sulphate and iron glycinate on SCFA concentration in broiler chickens’ caecum. Materials and methods: The feeding trial was carried out on 1-35-day old Ross 308 broiler chickens. 1000 broiler chickens were divided into 5 groups of 200 chickens each with 4 repeats in each group. The Group I (control) was fed with the standard compound feed. The experimental groups were fed the standard compound feed with additional iron supplementation (per kg of feed):Group II- 70 mg of iron sulphate and 72 mg of iron glycinate (after compound feed analysis 139 mg/kg); Group III - 144 mg of iron glycinate (after analysis 140 mg/kg); Group IV - 150 mg of iron sulphate (after analysis 147 mg/kg) and Group V - 72 mg of iron glycinate (after analysis 70 mg/kg). The broiler chickens were fed ad libitum. The content of SCFA in caecum was determined by high performance liquid chromatography method using ESC AccQ Tag technology (Waters Inc., USA). The obtained results were analysed using the one-way ANOVA test, and the significant differences between groups[...].