Surgical repair of chronic fistula in the dog using platelet-rich-fibrin (PRF) membrane

Abstract

Introduction Oronasal fistula is a congenital or acquired defe ct of mucosal communication between nasal and oral cavities. The most common complication of the surgery is palatal necrosis. Platelet rich fibrin membrane contains a fibrin matrix with leukocytes, erythrocytes, platelets, B and T lymphocytes, monocytes, s tem cells and growth factors which provides better and faster tissue regenerating. Clinical History 7 years old spayed female mix dog, weight 7.2 kg BMI=5, was presented for a dental cleaning procedure. During cautious physical examination an oronasal 3 c m wide fistula (3 cm mucous gap and 0,5 cm bone gap) was noticed on a rostral part of hard palate filled with purulent discharge. The owner noticed no clinical signs, except sneezing. Methods Procedure was done under general anesthesia. Using of bone graft was declined because of financial state of the owner. Oral cavity was cleaned with chlorhexydine 0.12% solution. Whole blood sample (5 ml) was collect from v. saphena into sterile tube without anticoagulant. WB sample was centrifuged on 1500 rpm, 14 min. After separation and compression, PRF membrane was put and fixated in the fistula between mucosa and periosteum of palatine process of incisive bone. Mucosal flap was created and sutured to close the fistula. Control visit was made 3 and 21 days after proc edure. Post op analgesia was 0.1 mg/kg meloxicam per os once a day, 3 days. For evaluation early wound healing ( and pain scale ( were used (Table 1). PRF may be a useful tool in oronasal fistula treatment but there is a need of more research about it s concept in dogs. [Table]. Table 1. CSR=clinical signs of re epitheliazation; CSH=clinical sigs of haemostasis; CSI=clinical signs of inflammation.