Antioxidant activity of different extracts and semisolid preparation containing propolis

Abstract

The aim of this research is to study antioxidative activity of liquid ethanol and oil propolis extracts and to produce semisolid preparatios adding of these extracts to Eucerin O/W emulsion basis. The total amount of polyphenol compounds in the extracts of propolis was determined using spectrophotometry. Pinocembrin and galangin, predominating propolis flavonoids, were chosen as a standard. Antioxidative activity was evaluated with the use of DPPH• stable free radical. The stability of propolis creams was investigated by visual method and centrifuging. The results of the study showed that thick extract of propolis is the richest in polyphenol compounds 6411.11 ± 421.67 mg/g PIN/GAL, oil extract of propolis – 23.99 ± 9.3 mg/g PIN/GAL and liquid 80 % ethanolic extract of propolis – 31.86 ± 1.99 mg/ml PIN/GAL. Although total polyphenolic amount was different all extracts of propolis showed high antioxidant activity: thick extract – 84.72 %, oil extract of propolis – 84.76 % and liquid extract of propolis – 72.94 % inactivated of free DPPH radical. Semisolid preparations with different extracts of propolis were produced by adding 0.5 – 3 % of each extract of propolis to Eucerin emulsion basis without the use of emulsifier. The homogeneity and stability of Eucerin creams with liquid ethanol propolis extract meet the general control requirements for semi-solid drug preparations. Eucerin creams with liquid 80 % ethanolic propolis extract showed higher (from 34.55 to 70.21 %) antioxidative activity comparing to Eucerin emulsion basis. Figure 1. Antioxidant actyvity of semisolid Eucerin emulsion containing liquid 80 % ethanolic extract of propolis.