The Biologically active compounds from tomato by-product obtained by SC-CO₂ technology and anti-inflammatory prioperties of extracts
| Author | Affiliation | |
|---|---|---|
Urbonavičienė, Dalia | ||
| Date |
|---|
2017-09-10 |
ISBN 978-609-02-1373-5.
Industrial vegetables, fruits and berries processing generates large amount of low-value by-products. There is the interest to use the biologically active compounds from plant original by-products to produced high value-added products in food, cosmetics, or pharmaceutical applications. Tomato by-products also represent a promising, low-cost source of biologically active compounds may be used in the end-products because of their favourable nutritional and technological properties. The aim of this study was to determine the biologically active compounds from tomato by -product obtained by supercritical carbon dioxide (SC-CO₂) technology and to determine the anti-inflammatory properties of the extracts in cell culture. The SC-CO₂ was applied to produce the biologically active compounds enriched oleoresin extract from tomato cv. "Admiro" F1 by-products. In this work, the recovery of biologically active compounds (carotenoids (especially lycopene and its isomers); fatty acid composition; the content of tocopherols) was obtained by SC-CO₂ technology. Carotenoids and its cis-isomers (lycopene and β-carotene) and the content of tocopherols in extract from tomato by-products were determined by high performance liquid chromatography (HPLC/DAD). The fatty acid composition of the lipid fraction obtained by SC-CO₂ at optimal conditions was analysed by gas chromatography. The anti-inflammatory activities of this extract were evaluated by assessing the production of hydrogen peroxide in a mouse's macrophage J774 cell culture. Results showed that the major fatty acids in tomato by-product extract isolated by SC-CO₂ were polyunsaturated linoleic acid (52±2%), followed by monounsaturated oleic (19±1%) acid. The concentration of the tocopherols was 3.01±0.004 g/kg. The extract was characterized for total β-carotene (63 mg/100g), lycopene (115 mg/100g) and lycopene different isomers (15-cis-lycopene; 13-cis-lycopene; 9-cis-lycopene; 7-cis-lycopene; trans. [...].