Application of thin layer chromatography and high performance thin layer chromatography in the analytical laboratory using the CAMAG analytical system

Abstract

Thin layer chromatography (TLC) is a chromatographic technique used to separate the components of a mixture using a thin stationary phase supported by an inert backing. It may be performed on the analytical scale as a means of monitoring the progress of a reaction, or on the preparative scale to purify small amounts of a compound. TLC is ananalytical tool widely used because of its simplicity, relative low cost, high sensitivity, and speed of separation. High performance thin-layer chromatography (HPTLC) is the most advanced form of TLC and comprises the use of chromatographic layers of utmost separation efficiency and the employment of state-of-the-art instrumentation for all steps in the procedure: precise sample application, standardized reproducible chromatogram development and software controlled evaluation. HPTLC is an entire concept that includes a widely standardized methodology based on scientific facts as well as the use of validated methods for qualitative and quantitative analysis. The aim of the presentation: to introduce the possibilities of the new generation CAMAG analytical system to perform TCL and HPTLC research. [...].