The Effect of Hyperoside Solution on Malondialdehyde (MDA) in Mice Brain

Abstract

Background. Hyperoside is one of the active components mostly found in H. perforatum L. Hyperoside has been associated with numerous biological activities that affect physiological processes of the organism. For example it is known to have antioxidant, anti-inflamatory and neuroprotective effects. Due to its antioxidant properties hyperoside is hypothesized to alleviate oxidative stress [1]. It is a condition when the balance between the production of free radicals in the body is disturbed. This condition can affect the damage of various organ systems, cells and DNA [2]. Malondialdehyde (MDA) is a traditional biomarker of oxidative stress, elevated MDA levels can indicate increased oxidative damage [3]. Aim. The aim of this study was to determine the effect of hyperoside solution to oxidative stress on MDA concentrations in the mice brain after AlCl3 exposure. Methods. Experiments were performed on 4 – 6 weeks old BALB/c mice. Mice were randomly apportioned to four groups with 8 mice per group – control, AlCl3, hyperoside and a group that received both aluminum (AlCl3) and hyperoside after 20 minutes. The mice were intragastrically administrated hyperoside solution for 21 days. MDA concentrations in the brain were determined using spectrophotometry at 535 and 520 nm. Results. Results showed that aluminum increased MDA concentrations in the brain of mice by 13 % compared to the control group (≥ 0,05). Hyperoside increased MDA concentration by 5% in comparison with control mice too (≥ 0,05). However, administration of hyperoside statistically significantly decreased MDA concentration in aluminium-treated mice brain by 54% compared to the aluminium-treated group (≤ 0,05). Conclusion. The ability of hyperoside to reduce elevated MDA concentration in the brain of mice after exposure of aluminum suggests that hyperoside has antioxidant properties. However, it would be appropriate to study the influence on the concentration of other oxidative stress markers as well, such as glutathione, catalase, etc.