In vitro cytotoxicity and antiviral activity of plant extracts on avian infectious bronchitis virus
| Author | Affiliation |
|---|---|
Mickienė, Rūta | |
Drevinskas, Tomas | |
Tiso, Nicola | |
| Date |
|---|
2016-06-20 |
EAN: 9789086862856 e-EAN: 9789086868315 ISBN: 978-90-8686-285-6 e-ISBN: 978-90-8686-831-5.
Avian infectious bronchitis (AIB) is an acute, highly contagious disease of major economic importance in commercial chicken flocks in Europe and Lithuania. Due to the low vaccination efficiency outbreaks of AIB virus often occurs in vaccinated flocks. One of the alternative methods for AIB prevention is the use of phytochemicals. The purpose of this work was the evaluation of cytotoxicity effect (CE) and screening for antiviral properties of medicinal plants and propolis extracts. Ethanol was used as control and 18 different extracts of medicinal plants and propolis were tested. Extracts were prepared using ethanol (40% vol.). Total amount of phenolic compounds, flavonoids and radical scavenging activity were determined spectrophotometrically. Composition of the phenolic compounds in the extracts was analysed chromatographically. CE was tested using Vero cells and Beaudette strain of AIB virus was used for antiviral properties testing. MTT assay was used for determination of CE. CE50 was calculated by linear regression analysis of the dose response curves. Inhibition of virus replication was evaluated by MTT assay and examination of the cells by light microscopy. Total amount of phenolic compounds varied in the extracts between 0.753-8.022 mg/ml, total amount of flavonoids between 0.041-2.486 mg/ml and radical scavenging activity between 0.326-13.47 mg/ml (expressed in rutin equivalents). The results of microscopy and MTT assay were correlated. Aqueous ethanol showed low CE while all plant and propolis extracts showed somewhat higher CE. Extract of Agastache foeniculum (Pursh) Kuntze showed the highest 6.32±0.13 log2 CE50, while extracts of Saturea montana L., Perilla frutescens L. Britton., Thymus vulgaris L. and Hyssopus officinalis L. showed the lowest 2.75±0.11, 2.70±0.30, 2.98±0.09, and 2.97±0.10 log2 CE50 respectively. The study of antiviral activity of extracts is in progress. The research showed that a[...].