Determination of protein quantity isolated from roots of Glycyrrhiza glabra L. and Desmodium canadense (L.) DC. during different vegetation phases

Abstract

Introduction Glycyrrhiza glabra L. and Desmodium canadense (L.) DC – are the herbaceous perennial species of Fabaceae family. Both plants are commonly used herbs in ayurvedic medicine. Studies have shown that extracts of plants roots possess antibacterial, antioxidant, antimalarial, anti-inflammatory properties. It was found that preparations of both plants contain a big amount of proteins, but the quantity has not been studied during different vegetation phases. Proteins are primary plant metabolites, which perform important vital functions [1;2;3]. The aim of the experiment - to compare protein content in liquorice and Canadian tick-trefoil roots during different vegetation phases – regrow, intensive growing, bud, blooming and seed maturity. Material and methods 1. Protein fractions were obtained from Glycyrrhiza glabra L. and Desmodium canadense (L.) DC raw root material by extraction and fractionation with ammonium sulphate and protease inhibitor aminocapronic acid. 2. The quantity of protein was measured by the Bradford method using bovine serum albumin BSA protein standards. 3. Results were analyzed with MS Excel. Results Proteins of Glycyrrhiza glabra L. and Desmodium canadense (L.) DC fresh root material was obtained in seven different vegetation phases. The biggest yield of protein was defined in liquorice root extract, while Canadian tick-trefoil root extract had a significant lower quantity of protein in all vegetation phases. Protein quantity in fractions extracted from liquorice roots material had a tendency to decline till massive blooming phase, from that point the output started to grow. At the end of the vegetation, protein amount has become approximately stable. Protein fractions, extracted from Canadian tick-trefoil roots, had the inclination to fluctuate. The biggest amount of protein was in seed maturity phase. Conclusions 1. The quantity of protein fractions amount was changing during vegetation in both extracts: the high