Influence of the inhalation with ethanol extract of propolis and honey on physiological parameters and inflammation in horses with chronic bronchitis

Abstract

Purpose of the work - The aim of the study was to examine the modulatory effect of ethanol extract of propolis (EEP) and honey inhalation on lung inflammatory function and physiological parameters in horses with bronchitis. Materials and used methods - 6 patients presented to LUHS Large Animal Clinic for bronchoscopy were included in the study. For all patient were done a clinical examination, bronchoscopy, taken tracheal aspirate (TA) for a bacteriological test before and after treatment. All horses were treated with EEP and honey inhalation 3 times per day for 7days. Propolis was extracted with 70% ethanol 1:10 (w/v). 1.4 ml of EEP plus 3.5 ml of honey for 500 kg/BW of the horse were mixed with 250 ml of 50 degrees of water and horse were inhaled using horse inhalator. After inhalation 40 ml of water mixed with 10 ml of EEP and 5 ml of honey were given per os. Outcomes - All horses had tachycardia and tachypnea before treatment. At the beginning of studies during bronchoscopy were found the mucus accumulation in the trachea 3.33 and in the bronchus was 3.33 (norm 0). Aspergillus spp., Bacillus spp., Penicillium spp., Pseudomonadales, Enterobacterales and Streptococcus spp. were isolated from TA of horses. In the 1 μl of TA were growing on average 199.83 colonies. After treatment, the heart rate and respiratory rate have decreased into the norm. The number of bacterial colonies in TA on 1 μl of TA has decreased until 24.47. Only Streptococcus spp. and Enterobacterales were isolated from TA of horses. The mucus accumulation in the trachea (0.33) and in the bronchus (1.33) was decreased. Conclusions - Inhalation with EEP in combination with honey for 7 days has shown antibacterial and antifungal action, heart and respiratory rates decreased action, and the mucolytic action in the lower respiratory tract in horses with chronic bronchitis.