Analysis of content of phenolic acids in Kazakhstanian and Lithuanian propolis ethanol extracts using high-performance liquid chromatography technique

Abstract

Introduction. Propolis is gaining popularity in the world market as a source of bioactive components. Characterization of each compound present in propolis is necessary to determine its biological activity (1). Propolis is a traditional medicine with known biological activities, and numerous studies have reported its versatile pharmacological activities, including antibacterial, antifungal, antiviral, anti-inflammatory, antioxidant and antitumor actions (2,3). Propolis is collectedin various countries – Greece, Turkey, Egypt, Croatia, Czech Republic, Bulgaria, Brasilia, Argentina, Chile, Russia, Taiwan, and Korea – and is analyzed using various methods of analysis (4). It is known that in Lithuanian predominant phenolic acids in propolis is ferulic and coumaric acids (4). The phenolic acids of Kazakhstanian propolis are still unknown. It is important to analyze the phenolic acids of Kazakhstanian propolis and to compare them with the phenolic acids of Lithuanian propolis. The study objective – to analyze phenolic acids in Kazakhstanian propolis ethanol extract and to compare it with the composition of Lithuanian propolis ethanol extract. The object. The ethanol extracts of Lithuanian and Kazakhstanian propolis. The sources of the raw material are presented in Table 1. Table 1. Propolis types 1 Lithuanian propolis UAB ,,Medicata’’ 2 Kazakhstan propolis Almaty area, Dzungaria region 3 Kazakhstan propolis East Kazakhstan region, the Altai city 4 Kazakhstan propolis Almaty area, Karbulak region 5 Kazakhstan propolis Almaty area, Alatajai region 6 Kazakhstan propolis Southern Kazakhstan Materials and methods. Experimental fluid extracts were produced from propolis by maceration method. The validated HPLC method was used to identify and to quantify phenolic acids (vanillic, caffeic, p-coumaric, ferulic acids) and vanillin. The quantity of phenolic compounds in propolis extract was determined using Agilent 8[...].