Regulation of bone marrow and airway CD34+ eosinophils by IL-5

Abstract

The aim of this study was to evaluate the effect of a neutralizing anti-IL-5 monoclonal antibody (TRFK-5) on bone marrow and airway CD34(+) and immature eosinophils. A focus was to determine to effect of the timing of treatment. Balb/c mice were OVA-sensitised and subsequently exposed to OVA from five to ten days via airway route. Animals were treated intraperitoneally with TRFK-5 or its isotype control (50 micro g) once at different time-points. Newly produced eosinophils were labelled using 5-bromo-2'-deoxyuridine (BrdU). BrdU(+) and CD34(+) eosinophil numbers were examined by immunocytochemistry. TRFK-5 reduced bone marrow immature eosinophils within three days. This effect was closely related to a reduction BrdU(+) and CD34(+) bone marrow eosinophils, and reduced numbers of blood eosinophils. However, BAL eosinophilia was not attenuated to the same degree. The effect of TRFK-5 was most prominent in the extended allergen-exposure protocol, where the treatment given in the middle of the exposure, with strongly reduced bone marrow CD34(+) and immature bone marrow eosinophils, blood eosinophils as well as BAL BrdU(+) eosinophils and BAL CD34(+) eosinophils. These data argue that anti-IL-5 down-regulates eosinophilopoiesis within three days by action in the bone marrow, by inhibition of the early stages of eosinophil maturation from CD34(+) cells.