Eosinophilopoietins receptors, TGF-ß1 and outer-membrane integrins expression of lung-resident and inflammatory eosinophils in severe non-allergic eosinophilic asthma
| Author | Affiliation |
|---|---|
Gosens, Reinoud | Department of Molecular Pharmacology, University of Groningen, Groningen, Netherlands |
| Date |
|---|
2019-09-28 |
6B Session 537 Poster discussion: Novel mechanisms elucidated by translational studies of asthma. Disease(s) : Airway diseases. Method(s) : Cell and molecular biology. Physiology. Pulmonary function testing. General respiratory patient care.
Introduction: Distinct eosinophils phenotypes, called lung-resident (rEOS) and inflammatory (iEOS), with different responses to eosinophilopoetins is recently found. However, there is no data about eosinophilopoetins receptors, growth factors and outer-membrane integrins expression in different EOS phenotypes in asthma. Aim: To investigate gene expression of IL-5, IL-3, GM-CSF receptors, TGF-β1 and α4β1, αMβ2 integrins in rEOS and iEOS during severe non-allergic eosinophilic asthma (SNEA). Methods: 7 SNEA patients with high dose of inhaled steroids and 7 healthy non-smokers (HS) were investigated. Blood EOS were isolated by high density centrifugation and magnetic separation, phenotyping-with antibodies against CD62L, conjugated with magnetic beads. Gene expression was measured by qPCR analysis. Results: In SNEA patients rEOS had significant upregulation of IL-3Rα by 8.1±4.3 folds, β1 integrin-5.1±2.1 folds and downregulation of αM integrin-18.7±4.9 folds, β2 integrin-3.2±1.3 folds and TGF-β1 -1.6±0.5 folds, comparing with iEOS (p<0.05). In SNEA group no significant differences was found in IL-5Rα, GM-CSFRα and α4 integrin genes expression. While in control group rEOS had upregulation of IL-3Rα by 1.6±0.6 folds, IL-5Rα -10.5±4.2 folds, GM-CSFRα -1.4±0.4 folds and α4 integrin -2.4±0.9 folds, comparing with iEOS (p<0.05). In HS group no significant differences was found in αM, β1, β2 integrins and TGF-β1 genes expression.