Mir-200a-3p Expression in the Serum of Patients with Parkinson's Disease

Abstract

Introduction Parkinson's disease (PD) is a neurodegenerative disorder that results in bradykinesia and tremor due to the death of dopaminergic neurons. PD progresses for several years before a clinical diagnosis is made and its treatment isnʻt curative [1]. Recently, miRNA (microRNA) interference has been extensively studied due to it’s affects in many biological processes, including manifestation of neurodegenerative diseases [2]. MiRNA molecules formed in various body cells can be packaged into extracellular vesicles (EV), then infiltrate the extracellular space and travel through biological fluids providing long lasting expression of disease – related genes [3]. EV miRNAs are expecting to help differentiate diseases, their stages and their progression. Aim The aim of this study was to determine EV miR-200a-3p expression in the serum of patients with PD. Methods MiRNA expression was evaluated by age, sex, the onset of the disease, its duration, severity ofsymptoms and selected method of treatment for 88 individuals with PD. 36 control group patients received medicational treatment, 39 deep brain stimulation and 13 gamma knife surgery. EV miRNAs were isolated from collected samples of blood serum, transcribed into cDNA and its expression was measured by RT-PCR. Statistical analysis was performed usingStudent's t test, ANOVA criteria and Pearsons correlation coefficient in GraphPad Software Inc.Prism 8. […].