Methylene blue ameliorates mitochondrial function of rat kidney during experimental acute pancreatitis

Abstract

Introduction. Systemic inflammatory response syndrome in severe acute pancreatitis (AP) leads to multiple organ dysfunction that is the main cause of death. Renal failure is of utmost importance and has a large impact on mortality rate. Our previous data revealed that mitochondrial energy metabolism in kidney is disturbed during experimental AP and may play an important role in the development and progression of renal failure. Objectives. The effects of methylene blue on mitochondrial respiratory functions of rat kidney during severe experimental AP were investigated in this study. Methods. AP was induced in 10 male Wistar rats by intraductal application of sodium taurocholate (5%, 1,75 mL/kg). Animals were divided into two groups: experimental group – methylene blue (5mg/kg) was injected intravenously 10 min prior to AP induction; control group – saline solution intravenously 10 min prior to AP induction. Animals were sacrificed after 24 hours from the induction of AP. Kidney were removed for mitochondrial assay by oxygraphic and spectrophotometric methods. [...].