Evaluation of cannabidiol and plant antioxidant activity dynamics in Lithuania cultivated Cannabis sativa L
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2015-05-14 |
Abstract speech of authors were not edited.
Cannabis sativa, depending on its breed and growth conditions may be used as a source of fiber, cannabis oil or as a medicinal plant [1]. The aim of this study was to determine cannabidiol (CBD) concentration and antioxidant activity dynamics of Cannabis sativa L. cultivated in Lithuania. The object of the study was Cannabis sativa L., Finola breed, female aerial plant parts (leaves, stems, flowers), which were collected in three different regions of Lithuania during four vegetation periods. Main biological substances in C. sativa L., which have attracted the most attention in science, are cannabinoids. Cannabidiol is neutral, non-psychotropic cannabinoid that distinguishes wide spectra of biological activities like: anticonvulsive, sedative, hypnotic, antipsychotic, anti-inflammatory and neuroprotective properties [2]. Beside cannabinoids, C.sativa L. contains plenty of terpenoids: monoterpenoids of which main are: β-myrcene, α-pinene, terpinolene and sesquiterpenoids of which main are: β-caryophyllene, caryophyllene oxide and humulene. [3] The latter substances are potential oxidative stress damage reducers [4]. Cannabidiol identification and concentration dynamics was determined using high-performance liquid chromatography method. Validation of quantitative assay of cannabidiol was performed. Antioxidant activity was evaluated using ferric reducing ability of plasma method and spectrophotometric analysis. Equivalent of antioxidant activity was 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid – otherwise known as trolox. The results have shown that the highest cannabidiol concentration was observed in flowers. The most high detected CBD concentration was 2,99 ± 0,22 mg/g which was determined in flowers, at the beginning of the flowering in Kupiškis region; the lowest cannbidiol amount was 0,024 ± 0,00001 mg/g in stems at the end of seed maturity in Kupiškis region. Results of antioxidant activity assay of Finola breed C. sativa L. have shown