Determination of bioactive compounds of Rhodiola rosea L.

Abstract

Introduction Rhodiola rosea L. in a well-known medicinal plant valued for its adaptogenic, antifatigue, antidiabetic, and neuroprotective effects (1). The key bioactive marker compounds are salidroside, rusavin, rosin and rosarian (2). However, the growing demand and climate change factors for golden roots have led to over-harvesting of the raw material and its inclusion in CITES (3). As the cultivation of Rhodiola expands to new regions, assessing the quality of its bioactive compounds is becoming increasingly important. The aim of this study was to choose method for identification of bioactive compounds in Rhodiola rosea L using high-performance liquid chromatography (HPLC) Materials and methods. were collected and introduced in Scientific Samples were collection of medicinal plants of Scientific Sector Modicinal (Aronsatic) Plams (MAP) of Department of Science at the Botanical Garden, Vytautas Magnus Magnus University. Kaunas, Lithuania. Analysis was conducted using Re proSil-Pur Basic C18 (150x4,6mm, particle size Jum) column and mobile phases of water, ncetonitrile and methanol, using gradient elution for 10 min (method A) In method B there was ACE 5 C18 (250x4.6mm, particle size 5 µm) and mobile phases were phosphatic buffer of pH7 with acetonitrile using gradient elution for 40 min. Detection for both methods was performed applying PDA detector at wavelength of 221 mm (salidroside) and 251 um (rosarin, rosavin, rosin, rosiridin). Results and discussion Both analysis methods can be used for detection of salidroside however, there were differences in retention times. Additionally, chromatograms showed variations in retention times and separation of rosavins (rosarin, cosavin, rosin, rosiridin). Method A proved to be more effective fise separating and detecting bioactive compounds due to the improved resolution of peaks in the chromatogram. 16 Conclusion The study reveals that HPLC analysis with Repro-Sil-Pur Basic CTB (150 fumm particle size 3um) column and mobile phases of water, acetonitrile and methanol provides shorter time of analysis, better separation of compounds leading to clearer chromatographic detection.