Effects of exposure to X-ray at airport security checkpoints on membrane integrity of chilled canine semen
| Author | Affiliation |
|---|---|
Šengaut, Jakov | Uždaroji akcinė bendrovė Jakovo veterinarijos centras |
| Date |
|---|
2016-10-06 |
ISBN 978-9955-15-448-8.
Konferencija skirta Lietuvos sveikatos mokslų universiteto Veterinarijos akademijos įkūrimo 80–čiui ir Lietuvos sveikatos mokslų universiteto Veterinarijos akademijos Gyvulininkystės technologijos fakulteto įkūrimo 70-čiui.
This study was conducted to investigate effects of X-irradiation on motility and viability of canine spermatozoids in chilled semen samples over the storage period of 5 days. Diluted semen samples from 10 different dogs were divided into 2 aliquots, and 1 group of aliquots was exposed to X-irradiation by transporting it through airport security X-ray machine (HI-SCAN 100100 T). Evaluation of total and progressive spermatozoids mitility was done by Sperm Class Analyzer (SCA) (Spain, 2011). To detect spermatozoa with a biochemically active plasma membrane, a hypo-osmotic swelling test was used. Assessment of sper atozoa with a structurally intact plasma membrane was done using SYBR-14/PI (Molecular Probes) fluorescent staining. Our study showed that the total and progressive mitility (P>0.05) and the percentage of canine spermatozoa with a functional (P≤0.0, P≤ 0.01) and intact (P>0.05) membrane over the storage period of 5 days were lower in the samples exposed to X-irradiation compared with the control group.