Use this url to cite publication: https://hdl.handle.net/20.500.12512/111829
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Effects of biguanides on mitochondrial permeability transition pore opening in intact brain cells / Cizas P, Stakauskas R, Borutaite V
Type of publication
Konferencijų tezės nerecenzuojamame leidinyje / Conference theses in non-peer-reviewed publication (T2)
Title
Effects of biguanides on mitochondrial permeability transition pore opening in intact brain cells / Cizas P, Stakauskas R, Borutaite V
Publisher (trusted)
Federation of European Neuroscience Societies |
Date Issued
Date Issued |
---|
2021-08-25 |
Extent
p. 111-111.
Is part of
Virtual Federation of European Neuroscience Societies (FENS) Regional Meeting : Kraków, Poland, 25-27 August 2021 : book of abstracts / Federation of European Neuroscience Societies. Polish Neuroscience Society (PNS). Lithuanian Neuroscience Association (LNA). [Brussels] : Federation of European Neuroscience Societies, 2021.
Version
Originalus / Original
Description
Poster session
Field of Science
Abstract
Mitochondrial permeability transition pore (MPTP) is thought to be involved in ischemia/reperfusion- -induced cell death in various organs, including the brain. Thus, inhibition of MPTP is considered as a target for neuroprotection. A biguanide compound metformin has been found to exert neuroprotective effects possibly involving modulation of mitochondrial functions. The aim of this study was to investigate the effects of various biguanide compounds - metformin, phenformin, imeglimin on ionomycin-induced MPTP opening in intact brain cells. Cultures of isolated rat neuronal and astrocytic cells were pre-treated with biguanides and were loaded with calcein-AM and CoCl2. Then ionomycin was added allowing entry of excess Ca2+ into cells and triggering MPTP opening which was observed as subsequent loss of mitochondrial calcein fluorescence measured with fluorescence microscope. We found that in neurons 2-3 mM metformin protected against ionomicin-induced MPTP opening. Phenformin exerted partial protective effect at 50 μM and at 100 μM concentration fully prevented MPTP opening. Imeglimin exerted partial protection at 1 μM and completely blocked MPTP opening at 10 μM concentration. In astrocytes, 2-3 mM metformin, 1-10 μM imeglimin and 50-100 μM phenformin partially suppressed ionomycin-induced MPTP opening. Our data suggest that metformin, phenformin and imeglimin prevent MPTP opening in intact neurons and astrocytes.
Type of document
type::text::conference output::conference proceedings::conference paper
Other Identifier(s)
(LSMU ALMA)990001044090107106
Coverage Spatial
Lenkija / Poland (PL)
Language
Anglų / English (en)
Funding(s)
Funding(s) | Grant No |
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European Social Fund under grant agreement with the Research Council of Lithuania. | No 09.3.3-LMT-K-712-01-0131 |