Assessment of genome stability of dogs

Abstract

Genome instabilities can lead to a wide variety of diseases and poor health parameters. Many endogenous and exogenous factors influence the level of damage to genetic material. Genome integrity depends on factors such as the fidelity of DNA replication, normal DNA organization in the chromosomes, and repair mechanisms. The aim of the study was to assess the stability of the genome of dogs using cytogenetic tests sister chromatid exchange, fragile sites, bleomycin, and comet. The study assessed the chromosome stability of Moloss oid dogs. Cytogenetic assays are very sensitive and can be used as biomarkers of normal DNA replication and repair potential and maintenance of control over the entire cell cycle. The use of these tests for a more accurate assessment of the genome stability and integrity of animals enable observation of the number of chromosomal instabilities generated in a given individual. The introduction of cytogenetic tests will enable a more precise assessment of the stability and integrity of the genome of animals and make it possible to observe the number of chromosomal instabilities generated in a given individual, which may be indicative of its health potential Genome instabilities can lead to a wide variety of diseases and poor health parameters. Many endogenous and exogenous factors influence the level of damage to genetic material. Genome integrity depends on factors such as the fidelity of DNA replication, normal DNA organization in the chromosomes, and repair mechanisms. The aim of the study was to assess the stability of the genome of dogs using cytogenetic tests sister chromatid exchange, fragile sites, bleomycin, and comet. The study assessed the chromosome stability of Moloss oid dogs. Cytogenetic assays are very sensitive and can be used as biomarkers of normal DNA replication and repair potential and maintenance of control over the entire cell cycle. The use of these tests for a more accurate assessment of the genome stability and integrity of animals enable observation of the number of chromosomal instabilities generated in a given individual. The introduction of cytogenetic tests will enable a more precise assessment of the stability and integrity of the genome of animals and make it possible to observe the number of chromosomal instabilities generated in a given individual, which may be indicative of its health potential.