Cellulose reinforced with hydroxyapatite for bone tissue engineering

Abstract

Celiuliozės gelis su hidroksiapatito intarpais gautas regeneruojant celiuliozę iš jos acetilintų darinių, palaipsniui įterpiant biokeramiką. Liofilizavus gelį gautas porėtas karkasas, kurio porėtumas siekia 75%. Nustatyta, kad kompozito porėtumas ir porų dydis gali būti reguliuojamas inkliuduojant mėginius organiniais tirpikliais prieš jų liofilizavimą. Atlikus bioaktyvumo testą in vitro parodyta, kad sintetiniame kūno skystyje ant karkaso formuojasi hidroksiapatitas. Introduction. Nowadays much attention is focused on natural polymer-ceramic three-dimensional scaffolds for bone tissue regeneration in defect size. Cellulose shares a number of requirements for scaffold material. It is an abundant renewable, biodegradable, non-toxic and biocompatible polymer. Moreover, it has similar mechanical properties compared to hard tissue. In this study cellulose reinforced with hydroxyapatite for the bone scaffold was investigated. Experimental. Cellulose scaffold was fabricated by regeneration of cellulose from its acetylated derivative adding hydroxyapatite. After the reaction the gel with homogeneously immobilized hydroxyapatite was obtained. In order to create suitable porous structure with nano-, micro-, macro- and gigapores the composite was freeze-dried. The porosity of the scaffold was determined by liquid displacement method. Bioactivity test in vitro was done by keeping the scaffold in simulated body fluid at 37°C. The X-ray differention (XRD), infrared spectroscopy (IR), and scanning electron microscopy (SEM) were used to characterize composite. [...].