Modernization of traditional poly-combination herbal medicinal product: development and validation of HPLC assay of the common markers quercetin, luteolin and emodin
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2012-11-24 |
Bibliogr.: p. 19
Trejos devynerios is a traditional herbal medicinal product with its origins going back to the times of the Both Nations Republic (1). This preparation was mentioned in the article Wiadomosci farmaceutycsne by prof. J. Muszynski in 1927 (2). In the 20th century, during the Soviet period, Trejos devynerios also known as ,trojanka’ was produced in Kaunas Pharmacy of Medicinal Herbs, in Poland company Herbapol produced ,Trojanka litewska’, in Chicago Lithuanian pharmacists working in J&J pharmacy, also labelled the similar polyingredient mixture as ,Trejos devynerios’ (3). Formula of this traditional phytopreparation includes 27 herbal drugs: poplar buds, capsicum, clove, oak bark, calendula flower, laurel leaf, peppermint leaf, dill fruit, hawthorn berries, wormwood, fragrant pepper, lovage root, valerian root, hop strobilus, bitter-orange epicarp and mesocarp, frangula bark, willow bark, caraway fruit, birch buds, meadowsweet, St. John's wort, hyssop, yarrow, thyme, tormentil rhizome, rowan berries, dog rose hip. Long-time use of this poly-ingredient preparation substantiates its phytotherapeutic properties; however, the substantial increase of quality requirements for herbal medicinal products brings new challenges to pharmaceutical science. The aim of the work: to select quality indicating common markers for assaying of product and to validate HPLC analytical method. Complexity of combination herbal medicinal product makes it difficult and, sometimes, impossible to give analytical identification and quantitative evaluation of the medicinal substances in the product (4). In these situations, with respect to scientific knowledge, application of combined markers, such as aglyconic fragments of various biologically active glycosidic forms is expedient. Hydrolysis of flavonoids and anthracene glycosides to aglycones is included in analytical method of the complicated product that contains 27 medicinal substances of plant origin. To optimise the analytical